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      A capillary electrophoresis method for genotyping the 9-bp exon 1 insertion/deletion in BDKRB2.

      Pharmacogenetics
      Electrophoresis, Capillary, methods, Exons, Genotype, Genotyping Techniques, Humans, INDEL Mutation, Polymorphism, Genetic, Receptor, Bradykinin B2, genetics

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          Abstract

          To develop and apply a novel genotyping method for the 9-bp exon 1 insertion/deletion polymorphism in BDKRB2. DNA from 718 patients with heart failure was extracted using standard methods and a region containing exon 1 of BDKRB2 was amplified with PCR. The PCR product was separated using the Qiagen QIAxcel® capillary electrophoresis system. The bp size of the PCR product was calculated and the genotypes determined using Qiagen BioCalculator® software. Capillary electrophoresis accurately genotyped samples with >99% call rate and 700 s run time per row of a 96-well plate (i.e., less than 1 min per sample). The frequency of the deletion was 49% in the Caucasian patients (n = 441) and 45% in the African-American (n = 277). Capillary electrophoresis is a rapid, accurate and sensitive method for genotyping the 9-bp exon 1 insertion/deletion polymorphism in BDKRB2.

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          Author and article information

          Journal
          22304584
          3290903
          10.2217/pgs.11.171

          Chemistry
          Electrophoresis, Capillary,methods,Exons,Genotype,Genotyping Techniques,Humans,INDEL Mutation,Polymorphism, Genetic,Receptor, Bradykinin B2,genetics

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