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      Colorimetric detection of hepatitis E virus based on reverse transcription loop mediated isothermal amplification (RT-LAMP) assay.

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          Abstract

          Hepatitis E virus (HEV) infection is a common communicable disease in the third world countries. The early diagnosis of HEV is important for the prognosis and prevention of the spread of the disease. Conventional methods for HEV detection need expensive equipment and cannot meet the needs of point of care (POC) use. In the present study, a gold nanoparticle (AuNP) based RT-LAMP assay was developed for colorimetric detection of HEV. As low as 10(1) copies of HEV RNA were detected visually. With reduced handling and less equipment requirement, this assay provided a simple and rapid alternative to other available methods.

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          Author and article information

          Journal
          J Virol Methods
          Journal of virological methods
          Elsevier BV
          1879-0984
          0166-0934
          Mar 2014
          : 197
          Affiliations
          [1 ] The 171 hospital of People's Liberation Army, 332000 Jiang xi, China.
          [2 ] People's Liberation Army, No 452 Hospital of Chengdu, 610021 Sichuan, China.
          [3 ] Chinese PLA 458th Hospital, 510602 Guangdong, China.
          [4 ] The 171 hospital of People's Liberation Army, 332000 Jiang xi, China. Electronic address: Dcy1111@163.com.
          Article
          S0166-0934(13)00476-X
          10.1016/j.jviromet.2013.11.006
          24269797
          71039e9a-139d-4b0e-ade8-f91daee405dd
          Copyright © 2013 Elsevier B.V. All rights reserved.
          History

          Colorimetric detection,Hepatitis E virus,Loop mediated isothermal amplification

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