Xiaojun Zhou 1 , 2 , 3 , 4 , 5 , Weizong Weng 6 , 7 , 8 , 9 , 10 , Bo Chen 11 , 12 , 13 , 14 , 10 , Wei Feng 1 , 2 , 3 , 4 , 5 , Weizhong Wang 1 , 2 , 3 , 4 , 5 , Wei Nie 1 , 2 , 3 , 4 , 5 , Liang Chen 1 , 2 , 3 , 4 , 5 , Xiumei Mo 1 , 2 , 3 , 4 , 5 , Jiacan Su 6 , 7 , 8 , 9 , 10 , Chuanglong He 1 , 2 , 3 , 4 , 5
A highly porous composite scaffold with localized and sustained antibiotic release property for treatment of infected bone defects.
Treatment of infected bone defects still remains a formidable clinical challenge, the design of bone implants with the controlled release of antibiotics is now regarded as a powerful strategy for infection control and bone healing. In this study, we fabricated a composite scaffold based on vancomycin (Van) loaded mesoporous silica nanoparticles (Van@MSNs) and a gelatin matrix. The microscopic structure of the gelatin-based composite scaffolds was characterized as highly porous. By the addition of MSNs, an enhancement in the compression property of MSNs-incorporated composite scaffolds was observed. The Van could release from the Van@MSNs incorporated composite scaffold in a sustained-release manner with a minimal burst, and thus effectively inhibit the growth of Staphylococcus aureus in a subsequent in vitro antibacterial study. In addition, the drug-loaded composite scaffold showed no unfavorable effects on the proliferation and differentiation of bone mesenchymal stem cells (BMSCs), confirming good biocompatibility. Moreover, in vivo results demonstrated that the antibiotic-loaded composite scaffold could significantly reduce bacterial contamination while promoting bone healing. Thus, our results suggest that the fabricated Van@MSNs/Gelatin composite scaffold with a localized and sustained release of antibiotics is a promising biomaterial for treating infected bone defects.