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      Design, deployment, and status of borehole observatory systems used for single-hole and cross-hole experiments, IODP Expedition 327, eastern flank of Juan de Fuca Ridge

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          Integrated Ocean Drilling Program (IODP) Expedition 327 installed two new subseafloor borehole observatory systems (“CORKs”) in 3.5 m.y. old upper ocean crust on the eastern flank of Juan de Fuca Ridge in Holes U1362A and U1362B. Expedition 327 participants also recovered part of an instrument string previously deployed in a CORK in Hole U1301B and deployed a short replacement string. These observatories are part of a network of six CORKs that was designed to monitor, sample, and complete multidisciplinary cross-hole experiments. We present an overview of project goals and describe the design, construction, and deployment of new CORK systems. We also provide an update on the status of preexisting CORK systems as of the start of Expedition 327. Additional CORK servicing and sampling are scheduled for summer 2011 and 2012, including a long-term free-flow perturbation experiment that will test the large-scale directional properties of the upper ocean crust around the observatories.

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            Methane-consuming archaea revealed by directly coupled isotopic and phylogenetic analysis.

            Microorganisms living in anoxic marine sediments consume more than 80% of the methane produced in the world's oceans. In addition to single-species aggregates, consortia of metabolically interdependent bacteria and archaea are found in methane-rich sediments. A combination of fluorescence in situ hybridization and secondary ion mass spectrometry shows that cells belonging to one specific archaeal group associated with the Methanosarcinales were all highly depleted in 13C (to values of -96 per thousand). This depletion indicates assimilation of isotopically light methane into specific archaeal cells. Additional microbial species apparently use other carbon sources, as indicated by significantly higher 13C/12C ratios in their cell carbon. Our results demonstrate the feasibility of simultaneous determination of the identity and the metabolic activity of naturally occurring microorganisms.
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              Composition of pore and spring waters from Baby Bare: global implications of geochemical fluxes from a ridge flank hydrothermal system


                Author and article information

                Proceedings of the IODP
                Integrated Ocean Drilling Program
                05 September 2011

                This work is licensed under a Creative Commons Attribution 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

                Self URI (journal page): http://publications.iodp.org/


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