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      A New Microbioassay for the Measurement of Lactogenic Hormones in Human Serum

      , ,

      Hormone Research in Paediatrics

      S. Karger AG

      Prolactin, Growth hormone, Microbioassay, Human serum

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          The standard Nb2 assay for biologically active prolactin has been modified to allow a rapid convenient microbioassay without loss of specificity or accuracy. Lactogenic hormones specifically stimulate the replication of Nb2 node rat lymphoma cells in suspension culture and form the basis of a currently available bioassay to measure prolactin and growth hormone in human serum. A new microbioassay was developed using microtest plates enabling a large number of samples to be assayed simultaneously whilst maintaining the overall sensitivity of the bioassay for lactogenic hormones. Growth of the Nb2 node lymphoma cells, measured by a light scattering technique using optical density on a spectrophotometer, was shown to be closely correlated with the cell number determined on a Coulter counter. Addition of excess anti-human prolactin and anti-human growth hormone completely inhibited the growth stimulatory effects of both human prolactin and human growth hormone. This new microbioassay (BA) and conventional radioimmunoassay (RIA) were used to measure lactogenic hormones in 48 normal subjects. There was a close correlation between the results of both assays for each hormone studied in the control sera. The mean basal BA/RIA ratio was 1.5 (range 0.8–2.0) for prolactin, 0.7 (range 0–4.5) for growth hormone and 1.3 (range 0.5–1.9) for total lactogenic activity.

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          Author and article information

          Horm Res Paediatr
          Hormone Research in Paediatrics
          S. Karger AG
          02 December 2008
          : 32
          : 5-6
          : 218-223
          University Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff, UK
          181293 Horm Res 1989;32:218–223
          © 1989 S. Karger AG, Basel

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          Page count
          Pages: 6
          Original Paper


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