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      MATE Transporter-Dependent Export of Hydroxycinnamic Acid Amides.

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          Abstract

          The ability of Arabidopsis thaliana to successfully prevent colonization by Phytophthora infestans, the causal agent of late blight disease of potato (Solanum tuberosum), depends on multilayered defense responses. To address the role of surface-localized secondary metabolites for entry control, droplets of a P. infestans zoospore suspension, incubated on Arabidopsis leaves, were subjected to untargeted metabolite profiling. The hydroxycinnamic acid amide coumaroylagmatine was among the metabolites secreted into the inoculum. In vitro assays revealed an inhibitory activity of coumaroylagmatine on P. infestans spore germination. Mutant analyses suggested a requirement of the p-coumaroyl-CoA:agmatine N4-p-coumaroyl transferase ACT for the biosynthesis and of the MATE transporter DTX18 for the extracellular accumulation of coumaroylagmatine. The host plant potato is not able to efficiently secrete coumaroylagmatine. This inability is overcome in transgenic potato plants expressing the two Arabidopsis genes ACT and DTX18. These plants secrete agmatine and putrescine conjugates to high levels, indicating that DTX18 is a hydroxycinnamic acid amide transporter with a distinct specificity. The export of hydroxycinnamic acid amides correlates with a decreased ability of P. infestans spores to germinate, suggesting a contribution of secreted antimicrobial compounds to pathogen defense at the leaf surface.

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          Author and article information

          Journal
          Plant Cell
          The Plant cell
          American Society of Plant Biologists (ASPB)
          1532-298X
          1040-4651
          Feb 2016
          : 28
          : 2
          Affiliations
          [1 ] Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany Interdisciplinary Centre for Crop Plant Research, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany.
          [2 ] Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany.
          [3 ] Interdisciplinary Centre for Crop Plant Research, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany Institute of Pharmacy, Biogenic Drugs, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany.
          [4 ] Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany.
          [5 ] Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany Interdisciplinary Centre for Crop Plant Research, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany srosahl@ipb-halle.de.
          Article
          tpc.15.00706
          10.1105/tpc.15.00706
          4790871
          26744218
          719c25b6-1741-4818-b4ac-5793a358b626
          History

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