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      Molecular and biological characterization of deformed wing virus of honeybees (Apis mellifera L.).

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          Abstract

          Deformed wing virus (DWV) of honeybees (Apis mellifera) is closely associated with characteristic wing deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult bees. The virus was purified from diseased insects, and its genome was cloned and sequenced. The genomic RNA of DWV is 10,140 nucleotides in length and contains a single large open reading frame encoding a 328-kDa polyprotein. The coding sequence is flanked by a 1,144-nucleotide 5' nontranslated leader sequence and a 317-nucleotide 3' nontranslated region, followed by a poly(A) tail. The three major structural proteins, VP1 (44 kDa), VP2 (32 kDa), and VP3 (28 kDa), were identified, and their genes were mapped to the N-terminal section of the polyprotein. The C-terminal part of the polyprotein contains sequence motifs typical of well-characterized picornavirus nonstructural proteins: an RNA helicase, a chymotrypsin-like 3C protease, and an RNA-dependent RNA polymerase. The genome organization, capsid morphology, and sequence comparison data indicate that DWV is a member of the recently established genus Iflavirus.

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          Author and article information

          Journal
          J Virol
          Journal of virology
          American Society for Microbiology
          0022-538X
          0022-538X
          May 2006
          : 80
          : 10
          Affiliations
          [1 ] Reparto di Biologia Moleculare, Istituto Zooprofilattico Sperimentale Lombardia e Emilia-Romagna, Brescia, Italy 25124-I.
          Article
          80/10/4998
          10.1128/JVI.80.10.4998-5009.2006
          1472076
          16641291
          72494853-f64d-4a3c-a096-210fa534675a
          History

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