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      Proteomic approaches in microalgae: perspectives and applications

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          Abstract

          Biofuels are the promising sources which are produced by various microalgae or in the form of metabolic by-products from organic or food waste products. Microalgae have been widely reported for the production of biofuels since these have a high storage of lipids as triacylglycerides, which can mainly be converted into biofuels. Recently, products such as biodiesel, bioethanol and biogas have renewed the interest toward the microalgae. The proteomics alone will not pave the way toward finding an ideal alga which will fulfill the current energy demands, but a combined approach of proteomics, genomics and bioinformatics can be pivotal for a sustainable solution. The present review emphasizes various technologies currently involved in algal proteomics for the efficient production of biofuels.

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          The Sorghum bicolor genome and the diversification of grasses.

          Sorghum, an African grass related to sugar cane and maize, is grown for food, feed, fibre and fuel. We present an initial analysis of the approximately 730-megabase Sorghum bicolor (L.) Moench genome, placing approximately 98% of genes in their chromosomal context using whole-genome shotgun sequence validated by genetic, physical and syntenic information. Genetic recombination is largely confined to about one-third of the sorghum genome with gene order and density similar to those of rice. Retrotransposon accumulation in recombinationally recalcitrant heterochromatin explains the approximately 75% larger genome size of sorghum compared with rice. Although gene and repetitive DNA distributions have been preserved since palaeopolyploidization approximately 70 million years ago, most duplicated gene sets lost one member before the sorghum-rice divergence. Concerted evolution makes one duplicated chromosomal segment appear to be only a few million years old. About 24% of genes are grass-specific and 7% are sorghum-specific. Recent gene and microRNA duplications may contribute to sorghum's drought tolerance.
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            Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production

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              Applications of genome-scale metabolic reconstructions

              The availability and utility of genome-scale metabolic reconstructions have exploded since the first genome-scale reconstruction was published a decade ago. Reconstructions have now been built for a wide variety of organisms, and have been used toward five major ends: (1) contextualization of high-throughput data, (2) guidance of metabolic engineering, (3) directing hypothesis-driven discovery, (4) interrogation of multi-species relationships, and (5) network property discovery. In this review, we examine the many uses and future directions of genome-scale metabolic reconstructions, and we highlight trends and opportunities in the field that will make the greatest impact on many fields of biology.
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                Author and article information

                Contributors
                pratyoosh.shukla@gmail.com
                Journal
                3 Biotech
                3 Biotech
                3 Biotech
                Springer Berlin Heidelberg (Berlin/Heidelberg )
                2190-572X
                2190-5738
                30 June 2017
                July 2017
                : 7
                : 3
                : 197
                Affiliations
                [1 ]ISNI 0000 0004 1790 2262, GRID grid.411524.7, Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, , Maharshi Dayanand University, ; Rohtak, 124001 Haryana India
                [2 ]ISNI 0000 0001 2184 3953, GRID grid.417984.7, Department of Environmental Science and Engineering, , Indian Institute of Technology (Indian School of Mines), ; Dhanbad, Jharkhand India
                Article
                831
                10.1007/s13205-017-0831-5
                5493573
                28667637
                7297f0e1-c419-46d6-bb76-3f752d1368d7
                © Springer-Verlag GmbH Germany 2017
                History
                : 17 February 2017
                : 19 May 2017
                Categories
                Review Article
                Custom metadata
                © Springer-Verlag GmbH Germany 2017

                microalgae,biofuel,proteomics,itraq,systems biology
                microalgae, biofuel, proteomics, itraq, systems biology

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