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      The zebrafish IgH locus contains multiple transcriptional regulatory regions.

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          Abstract

          Many fish have, in addition to IgM and IgD, a third isotype called IgZ or IgT. The ζ-chain locus is embedded among the Ig heavy chain V-, D- and J-elements in a manner reminiscent of the TcR δ/α locus. Isotype selection thus occurs during VDJ recombination, a process that is facilitated by intralocus transcription. Using in silico analyses and enhancer reporter vectors we identified 3 new regions within the zebrafish IgH locus through which transcription can be activated in catfish B-cell lines. Two of these, termed Eζi (Jζ to Cζ1 intronic) and Eζ3' regions flank the ζ-chain constant domain exons. A third region, Eδ3', resides downstream of the δ-chain exons. All regions contain predicted binding sites for transcription factors that contribute to B-cell specific transcription in fish and mammals. Each region also has proximal matrix attachment regions, which may further contribute to transcriptional activation and chromatin remodeling. We discuss possible roles for these regions during VDJ recombination.

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          Author and article information

          Journal
          Dev. Comp. Immunol.
          Developmental and comparative immunology
          Elsevier BV
          1879-0089
          0145-305X
          Mar 2011
          : 35
          : 3
          Affiliations
          [1 ] Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. ndanilova@gmail.com
          Article
          S0145-305X(10)00256-9 NIHMS251263
          10.1016/j.dci.2010.10.010
          3031712
          21055416

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