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      Recombinogenic telomeres in diploid Sorex granarius (Soricidae, Eulipotyphla) fibroblast cells.

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          Abstract

          The telomere structure in the Iberian shrew Sorex granarius is characterized by unique, striking features, with short arms of acrocentric chromosomes carrying extremely long telomeres (up to 300 kb) with interspersed ribosomal DNA (rDNA) repeat blocks. In this work, we investigated the telomere physiology of S. granarius fibroblast cells and found that telomere repeats are transcribed on both strands and that there is no telomere-dependent senescence mechanism. Although telomerase activity is detectable throughout cell culture and appears to act on both short and long telomeres, we also discovered that signatures of a recombinogenic activity are omnipresent, including telomere-sister chromatid exchanges, formation of alternative lengthening of telomeres (ALT)-associated PML-like bodies, production of telomere circles, and a high frequency of telomeres carrying marks of a DNA damage response. Our results suggest that recombination participates in the maintenance of the very long telomeres in normal S. granarius fibroblasts. We discuss the possible interplay between the interspersed telomere and rDNA repeats in the stabilization of the very long telomeres in this organism.

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          Author and article information

          Journal
          Mol. Cell. Biol.
          Molecular and cellular biology
          American Society for Microbiology
          1098-5549
          0270-7306
          Aug 2014
          : 34
          : 15
          Affiliations
          [1 ] Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia zhdanova@bionet.nsc.ru Arturo.Londono@curie.fr.
          [2 ] Telomere & Cancer Laboratory, Equipe Labelisée Ligue Nationale contre le Cancer, Institut Curie, Paris, and UPMC University Paris, Paris, France.
          [3 ] Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
          [4 ] Moscow State University, Chemical Faculty, Moscow, Russia.
          [5 ] Telomere & Cancer Laboratory, Equipe Labelisée Ligue Nationale contre le Cancer, Institut Curie, Paris, and UPMC University Paris, Paris, France zhdanova@bionet.nsc.ru Arturo.Londono@curie.fr.
          Article
          MCB.01697-13
          10.1128/MCB.01697-13
          4135569
          24842907
          72f0772c-ef3c-40a6-83d2-2710ff19ef08

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