0
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Isolation and Characterization of Soluble and Particulate Thyroid Iodoproteins in Human Congenital Goiter

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Soluble and particulate iodoproteins solubilized by detergent from in vivo <sup>125</sup>I labeled congenital goiter, have been isolated and characterized. The soluble iodoproteins (S<sub>1</sub>) were decreased but contained 80% of radioiodine; 19S thyroglobulin (TG) represented only 9% of the soluble extract, the main component being iodinated serum albumin. The solubilized iodoproteins (S<sub>2</sub>) consisted of TG-like material and represented one third of the total TG, sedimented as 19S and possessed the immunoreactive properties of native TG. The iodine content of TG from S<sub>1</sub> and S<sub>2</sub> was 0.01% and 0.008%, respectively, and no radio-labeled truodothyronine or tetraiodothyronine was detectable in the two TG containing only monoiodotyrosine and diiodotyrosine. S<sub>1</sub> and S<sub>2</sub> TG showed the same resistance to dissociation at alkaline pH in low salt. The sialic acid content was markedly decreased in both S<sub>1</sub> and S<sub>2</sub> TG, the particulate protein contained 0.06%, compared to 0.12% found in the soluble goitrous fraction and to 1.3% described in the normal soluble TG. The results presented, whilst confirming the striking decrease of soluble TG and the increase in the particulate iodoproteins, lend support to the hypothesis that TG synthesis is impaired in some cases of human congenital goiter. Moreover, our findings demonstrate the presence of membrane-bound 19S molecules and a decreased sialic acid content in the goitrous TG, which is more marked in the particulate fraction. These results suggest that a defective secretion into the follicular lumen of fully synthesized TG could be responsible for the dishormonogenetic errors in some cases of congenital goiter.

          Related collections

          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1974
          1974
          21 November 2008
          : 5
          : 3
          : 141-155
          Affiliations
          Centro di Fisiopatologia Tiroidea (CNR), 2 a Clinica Medica, Università di Roma, Rome
          Article
          178625 Horm Res 1974;5:141–155
          10.1159/000178625
          4209110
          © 1974 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 15
          Categories
          Paper

          Comments

          Comment on this article