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      Epidermal growth factor as an autocrine modulator of stress response in mammary epithelial cells.

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      The Journal of endocrinology

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          Abstract

          Mouse mammary epithelial cells (NMuMG line) were heat shocked at 42 degreesC for up to 2 h, with continuous culture at 37 degreesC used as controls. EGF mRNA expression, determined by Northern blot hybridization, was increased 4- to 5-fold in heat-shocked cells compared with controls. Heat shock also induced accumulation of EGF immunoreactive protein in both media and cell lysates of NMuMG cells. Oligonucleotides antisense to the EGF mRNA blocked the accumulation of EGF protein, while sense controls were without effect. Antisense oligonucleotide concentrations that inhibited EGF production by NMuMG cells during heat shock dramatically reduced the ability of cells to survive heat shock, while sense oligonucleotide did not affect cell survival. The antisense oligonucleotide inhibition of cell survival was reversed by adding EGF during the heat shock period. Thus the production of EGF or EGF-like peptides during periods of cellular stress may be an important survival mechanism in mammary epithelium.

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          Author and article information

          Journal
          J. Endocrinol.
          The Journal of endocrinology
          0022-0795
          0022-0795
          Oct 1998
          : 159
          : 1
          Affiliations
          [1 ] Department of Dairy Science, 266 Animal Sciences Building, University of Wisconsin, Madison, WI 53706, USA.
          Article
          9795348
          73c05c0e-b0fe-4090-afc7-68a14f39cd09
          History

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