To examine the effect of neuropeptide Y (NPY) on TGF-β1 production in RAW264.7 macrophages.
Enzyme linked immunosorbent assay (ELISA) was used to detect TGF-β1 production. Cell counting kit 8 (CCK-8) was used to assay the viability of RAW264.7 cells. Western blot was used to detect the phosphorylation of PI3K p85.