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Primers for mating-type diagnosis in Diaporthe and Phomopsis: their use in teleomorph induction in vitro and biological species definition
Author(s):
Jorge M. Santos
,
Viviana G. Correia
,
Alan J.L. Phillips
Publication date
Created:
February 2010
Publication date
(Print):
February 2010
Journal:
Fungal Biology
Publisher:
Elsevier BV
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Abstract
Sexual reproduction in ascomycete fungi is governed by the mating-type (MAT) locus. The MAT loci of Diaporthe and its Phomopsis anamorphs differ in only one gene: MAT1-1-1 in mating-type MAT1-1 and MAT1-2-1 in mating-type MAT1-2. In order to diagnose mating-types in Diaporthe and Phomopsis and evaluate their usefulness in teleomorph induction in vitro and biological species delimitation, we designed primers that amplify part of the MAT1-1-1 and MAT1-2-1 genes. MAT phylogenies were generated and compared with ITS and EF1-α phylograms. Species recognised in the EF1-α phylogeny corresponded directly with those determined in the MAT phylogenies. ITS was shown to be highly variable resulting in a large number of phylogenetic species that were discordant with MAT and EF1-α species. Mating experiments were conducted to evaluate the existence of reproductive barriers between some isolates, and their anamorphic morphologies were compared. The primers proved to be useful in the mating-type diagnosis of isolates, selection of compatible mating pairs, and in the assessment of biological species boundaries. Copyright © 2010 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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Inflamed lung in vitro co-culture models
Author and article information
Journal
Title:
Fungal Biology
Abbreviated Title:
Fungal Biology
Publisher:
Elsevier BV
ISSN (Print):
18786146
Publication date Created:
February 2010
Publication date (Print):
February 2010
Volume
: 114
Issue
: 2-3
Pages
: 255-270
Article
DOI:
10.1016/j.funbio.2010.01.007
PubMed ID:
20943136
SO-VID:
74b07780-807c-4bc9-a6a9-bf9caed52618
Copyright ©
© 2010
License:
https://www.elsevier.com/tdm/userlicense/1.0/
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