Blog
About

7
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Charge-coupled-device based scanner for tomography of fluorescent near-infrared probes in turbid media.

      Medical physics

      Biophysical Phenomena, statistics & numerical data, instrumentation, Tomography, Spectroscopy, Near-Infrared, Photons, Optics and Photonics, Nephelometry and Turbidimetry, Image Processing, Computer-Assisted, Humans, Fluorescent Dyes, Equipment Design, Biophysics

      Read this article at

      ScienceOpenPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          We present a novel tomographer for three-dimensional reconstructions of fluorochromes in diffuse media. Photon detection is based on charge-coupled device technology that allows the implementation of a large parallel array of detection channels with high sensitivity. Using this instrument we studied the response and detection limits of near-infrared fluorochromes in diffuse media as a function of light intensity and for a wide range of biologically relevant concentrations. We further examined the resolution of the scanner and the reconstruction linearity achieved. We demonstrate that the instrument attains better than 3 mm resolution, is linear within more than two orders of magnitude of fluorochrome concentration, and can detect fluorescent objects at femto-mole quantities in small animal-like geometries. These measurements delineate detection and reconstruction characteristics associated with imaging of novel classes of fluorescent probes developed for in vivo molecular and functional probing of tissues.

          Related collections

          Author and article information

          Journal
          12033576

          Comments

          Comment on this article