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      Scale-up micropropagation of sweet basil (Ocimum basilicum L.) in an airlift bioreactor and accumulation of rosmarinic acid.

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          Abstract

          Nodal explants with lateral buds and leaf-derived suspension cultures of sweet basil, Ocimum basilicum L., were cultured in 5 l airlift bioreactors for three weeks, thereby increasing the fresh wt of suspension cultures 2.5-fold. Rosmarinic acid accumulated at 29 micromicrog g(-1) dry wt in the suspension culture but, for micropropagated plants, it reached 178 microg g(-1) dry wt.

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          Author and article information

          Journal
          Biotechnol Lett
          Biotechnology letters
          Springer Science and Business Media LLC
          0141-5492
          0141-5492
          Mar 2004
          : 26
          : 6
          Affiliations
          [1 ] Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece. skin@aua.gr
          Article
          10.1023/b:bile.0000019561.89044.30
          15127795
          7526e062-19ff-4497-bec6-ee2e792b2f70
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