A β1-tubulin–based megakaryocyte maturation reporter system identifies novel drugs that promote platelet production

Abstract

<p id="d8531319e277"> <div class="list"> <a class="named-anchor" id="d8531319e279"> <!-- named anchor --> </a> <ul class="so-custom-list"> <li id="d8531319e280"> <div class="so-custom-list-content so-ol"> <p class="first" id="d8531319e281">β1-tubulin–Venus reporter megakaryocyte cell line enabled high-throughput drug screening by monitoring megakaryocyte maturation. </p> </div> </li> <li id="d8531319e283"> <div class="so-custom-list-content so-ol"> <p class="first" id="d8531319e284">The identified compounds, Wnt-C59 and TCS 359, induced megakaryocyte maturation under feeder-free conditions through distinct mechanisms. </p> </div> </li> </ul> </div> </p><p class="first" id="d8531319e288">During maturation, megakaryocytes (MKs) express β1-tubulin (TUBB1) and rearrange their microtubule components to enlarge, form proplatelets, and eventually release platelets. The development of a platform to identify in vitro conditions that would efficiently promote MK development could potentially enable large-scale platelet production. Here, we show that an immortalized MK cell line (imMKCL) genetically modified to express the β1-tubulin–Venus reporter provides a practical system to efficiently monitor the in vitro production of platelet-like particles (PLPs). The Venus transgene was inserted downstream of the <i>TUBB1</i> locus in imMKCLs using CRISPR/Cas9, and the expression was visualized by Venus fluorescence intensity. This imMKCL reporter line was then used for high-throughput drug screening. We identified several compounds that significantly improved the efficiency of PLP production in vitro under feeder-free conditions and showed a significant tendency to recover platelets in vivo in a mouse thrombocytopenia model induced by anti-GPIbα antibody administration. Interestingly, most of these compounds, including a WNT signaling pathway inhibitor, Wnt-C59, antagonized the aryl hydrocarbon receptor (AhR) to increase PLP production, confirming the crucial role of AhR inhibition in MK maturation. Consistently, small interfering RNA treatment against AhR increased the Venus intensity and PLP production. TCS 359, an FLT3 inhibitor, significantly increased PLP production independently of FLT3 or AhR. This study highlights the usefulness of the β1-tubulin reporter MK line as a useful tool to study the mechanisms underlying thrombopoiesis and to identify novel inducers of ex vivo platelet production. </p><p id="d8531319e297"> <div class="fig panel" id="absf1"> <a class="named-anchor" id="absf1"> <!-- named anchor --> </a> <div class="figure-container so-text-align-c"> <img alt="" class="figure" src="/document_file/576ea624-0ae4-4c2f-902c-3cb6429f7364/PubMedCentral/image/advances019547absf1"/> </div> <div class="panel-content"/> </div> </p>