For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
57
views
13
references
 Top references
cited by
11
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      294
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Evaluation of a New Immunochromatographic Assay Kit for the Rapid Detection of Norovirus in Fecal Specimens

      brief-report

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Rapid and accurate detection of norovirus is essential for the prevention and control of norovirus outbreaks. This study compared the effectiveness of a new immunochromatographic assay kit (SD BIOLINE Norovirus; Standard Diagnostics, Korea) and real-time reverse transcription-PCR (RT-PCR) for detecting norovirus in fecal specimens. Compared with real-time RT-PCR, the new assay had sensitivity, specificity, positive predictive value, and negative predictive value of 76.5% (52/68), 99.7% (342/343), 98.1% (52/53), and 95.5% (342/358), respectively. The sensitivity of the assay was 81.8% (18/22) for GII.3 and 75.7% (28/37) for GII.4. None of the 38 enteric virus-positive specimens (3 for astrovirus, 5 for enteric adenovirus, and 30 for rotavirus) tested positive in the cross-reactivity test performed by using this assay. The new immunochromatographic assay may be a useful screening tool for the rapid detection of norovirus in sporadic and outbreak cases; however, negative results may require confirmatory assays of greater sensitivity.

          Related collections

          Most cited references13

          • Record: found
          • Abstract: not found
          • Article: not found

          Noroviruses: a comprehensive review.

          Manish M Patel, Aron Hall, Jan Vinjé … (2009)
          Article 0 comments Cited 212 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Diagnostic accuracy and analytical sensitivity of IDEIA Norovirus assay for routine screening of human norovirus.

            Antony Hale, Jan Vinjé, Christianne Biggs … (2010)
            Noroviruses (NoVs) are recognized as the leading cause of epidemic and sporadic acute gastroenteritis. Early detection of NoV is crucial to control the spread of the disease. In this study, we evaluated the diagnostic accuracy, analytical sensitivity, and analytical reactivity of the IDEIA Norovirus assay (an enzyme immunoassay [EIA]) in a prospective and retrospective study design. A total of 557 prospectively collected fecal samples and a panel of 97 archived fecal samples, including 21 different GI and GII genotypes, were tested by conventional reverse transcription-PCR (RT-PCR)/bidirectional sequencing, real-time RT-PCR, and electron microscopy. The sensitivity and specificity of the EIA were 57.6% and 91.9%, respectively. The sensitivity for detecting NoV in fecal samples from outbreaks improved from 44.1% when three samples were tested to 76.9% when five samples per outbreak were tested. The EIA was able to detect strains from 7 GI and 11 GII genotypes. The analytical sensitivity of the EIA was 3.1 x 10(6) and 1.6 x 10(7) virus particles g(-1) of fecal sample for NoV GI and GII strains, respectively. Most GII samples positive by EIA had a threshold cycle (C(T)) of 25.6, suggesting that, although strains from genotypes GI.8, GII.10, and GII.16 were not detected, the low sensitivity of the EIA is primarily caused by low virus concentration. In conclusion, the current EIA may be of use as a rapid screening test during a norovirus outbreak investigation when multiple fecal samples are available; however, sporadic samples should be tested by molecular methods.
            Article 0 comments Cited 22 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Caliciviridae: The noroviruses

              Green, KY Green, K Green (2007)
              Article 0 comments Cited 21 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Ann Lab Med
                Journal ID (publisher-id): ALM
                Title: Annals of Laboratory Medicine
                Publisher: The Korean Society for Laboratory Medicine
                ISSN (Print): 2234-3806
                ISSN (Electronic): 2234-3814
                Publication date (Print): January 2012
                Publication date (Electronic): 20 December 2011
                Volume: 32
                Issue: 1
                Pages: 79-81
                Affiliations
                [1 ]Chungcheongnam-Do Health and Environment Research Institute, Daejeon, Korea.
                [2 ]Daejeon Health and Environment Research Institute, Daejeon, Korea.
                [3 ]Department of Pediatrics, College of Medicine, Soonchunhyang University, Cheonan, Korea.
                [4 ]Department of Clinical Parasitology and Allergy, College of Medicine, Soonchunhyang University, Cheonan, Korea.
                [5 ]Department of Biochemistry, College of Medicine, Soonchunhyang University, Cheonan, Korea.
                [6 ]Department of Laboratory Medicine, College of Medicine, Soonchunhyang University, Cheonan, Korea.
                Author notes
                Corresponding author: Young Jin Choi. Department of Laboratory Medicine, Soonchunhyang University Cheonan Hospital, 23-20 Bongmyung-dong, Cheonan, 330-721, Korea. Tel: +82-41-570-3562, Fax: +82-41-572-2316, clinpath@ 123456sch.ac.kr
                Article
                DOI: 10.3343/alm.2012.32.1.79
                PMC ID: 3255496
                PubMed ID: 22259783
                SO-VID: 75740882-5050-4163-a51c-37ae8d4c3538
                Copyright © © The Korean Society for Laboratory Medicine
                License:

                This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 09 June 2011
                Date revision received : 11 July 2011
                Date accepted : 16 August 2011
                Categories
                Subject: Brief Communication
                Subject: Clinical Microbiology

                ScienceOpen disciplines: Clinical chemistry
                Keywords: norovirus,sensitivity,specificity,immunochromatographic assay
                Data availability:
                ScienceOpen disciplines: Clinical chemistry
                Keywords: norovirus, sensitivity, specificity, immunochromatographic assay

                Comments

                Comment on this article

                scite_

                Similar content294

                See all similar

                Cited by10

                See all cited by

                Most referenced authors103

                See all reference authors