Blog
About

  • Record: found
  • Abstract: found
  • Article: found
Is Open Access

Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide

Read this article at

Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Abstract

      Lonicera japonica is a typical Chinese herbal medicine. We previously reported a method to isolate polysaccharides from Lonicera japonica (LJP). In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method. We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models. The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides. In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice. LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice. Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective. Therefore, LJP could be used as a potential immunomodulatory agent.

      Related collections

      Most cited references 40

      • Record: found
      • Abstract: found
      • Article: not found

      Effects of spaceflight on innate immune function and antioxidant gene expression.

      Spaceflight conditions have a significant impact on a number of physiological functions due to psychological stress, radiation, and reduced gravity. To explore the effect of the flight environment on immunity, C57BL/6NTac mice were flown on a 13-day space shuttle mission (STS-118). In response to flight, animals had a reduction in liver, spleen, and thymus masses compared with ground (GRD) controls (P < 0.005). Splenic lymphocyte, monocyte/macrophage, and granulocyte counts were significantly reduced in the flight (FLT) mice (P < 0.05). Although spontaneous blastogenesis of splenocytes in FLT mice was increased, response to lipopolysaccharide (LPS), a B-cell mitogen derived from Escherichia coli, was decreased compared with GRD mice (P < 0.05). Secretion of IL-6 and IL-10, but not TNF-alpha, by LPS-stimulated splenocytes was increased in FLT mice (P < 0.05). Finally, many of the genes responsible for scavenging reactive oxygen species were upregulated after flight. These data indicate that exposure to the spaceflight environment can increase anti-inflammatory mechanisms and change the ex vivo response to LPS, a bacterial product associated with septic shock and a prominent Th1 response.
        Bookmark
        • Record: found
        • Abstract: found
        • Article: not found

        Immune dysfunction in cirrhosis: Distinct cytokines phenotypes according to cirrhosis severity.

        Cirrhosis associated immune dysfunction has been proposed to switch from a pro-inflammatory phenotype in stable cirrhosis to an immunodeficient one in patients with decompensated cirrhosis and acute-on-chronic liver failure. The aim of the present study was to compare serum cytokine levels between healthy patients, stable cirrhosis, and decompensated cirrhotic patients with and without development of acute-on-chronic liver failure (ACLF); and to explore whether any of the measured cytokines is associated with cirrhosis severity and prognosis in ACLF patients.
          Bookmark
          • Record: found
          • Abstract: found
          • Article: not found

          FT-IR investigation of cell wall polysaccharides from cereal grains. Arabinoxylan infrared assignment.

          The FT-IR fingerprint of wheat endosperm arabinoxylan (AX) was investigated using a set of polysaccharides exhibiting variation of their degree of substitution and xylo-oligosaccharides comprising xylose units mono- or disubstituted by arabinose residues. Substitution of the xylose backbone by arabinose side units was more particularly studied in the 1000-800 cm(-1) spectral region, by taking advantage of second-derivative enhancement. The 920-1020 cm(-1) spectral region revealed two absorption bands at 984 and 958 cm(-1), the intensities of which varied according to the degree of substitution. Whereas the intensity of the band at 958 cm(-1) increased with the degree of substitution, that at 984 cm(-1) decreased. The second-derivative spectral data of xylo-oligosaccharides indicated that these changes could be attributed to substitution of the xylan backbone by arabinose residues, and the band at 958 cm(-1) was ascribed to the presence of disubstituted xylose residues. Principal component analysis of FT-IR spectra of model mixtures of AX, beta-glucans, and arabinogalactans suggested that it is possible to evaluate the relative proportions of the polymers and degree of substitution of AX in complex mixtures such as the cell wall of cereal grains.
            Bookmark

            Author and article information

            Affiliations
            [1 ] Key Laboratory of Polysaccharide Drug Engineering of Anhui, Wannan Medical College, Wuhu, Anhui, P. R. China
            [2 ] College of Life Sciences, Anhui Normal University, Wuhu, P. R. China
            [3 ] College of Information Engineering, Changsha Medical University, Changsha, Hunan, P. R. China
            [4 ] College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, P. R. China
            [5 ] Department of Mathematics, Shanghai Normal University, Shanghai, P. R. China
            [6 ] Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America
            University of Manchester, UNITED KINGDOM
            Author notes

            Competing Interests: The authors have declared that no competing interests exist.

            Contributors
            Role: Methodology, Role: Validation, Role: Writing – original draft
            Role: Data curation, Role: Investigation, Role: Writing – review & editing
            ORCID: http://orcid.org/0000-0002-9225-407X, Role: Data curation, Role: Resources, Role: Writing – review & editing
            Role: Data curation, Role: Methodology, Role: Writing – review & editing
            Role: Data curation, Role: Methodology, Role: Writing – review & editing
            Role: Conceptualization, Role: Funding acquisition, Role: Investigation, Role: Writing – review & editing
            ORCID: http://orcid.org/0000-0003-4689-8672, Role: Conceptualization, Role: Funding acquisition, Role: Writing – original draft, Role: Writing – review & editing
            Role: Editor
            Journal
            PLoS One
            PLoS ONE
            plos
            plosone
            PLoS ONE
            Public Library of Science (San Francisco, CA USA )
            1932-6203
            8 October 2018
            2018
            : 13
            : 10
            30296293 6175272 10.1371/journal.pone.0204152 PONE-D-18-16870
            © 2018 Zhou et al

            This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

            Counts
            Figures: 6, Tables: 3, Pages: 15
            Product
            Funding
            Funded by: The National Science Foundation of China
            Award ID: No. 11201306 and No. 61572327
            Award Recipient :
            Funded by: the Innovation Program of Shanghai Municipal Education Commission
            Award ID: No. 13YZ065
            Award Recipient :
            Funded by: the Natural Science Foundation of Hunan, China
            Award ID: No. 2018JJ2461
            Award Recipient : ORCID: http://orcid.org/0000-0003-4689-8672
            This work was financially supported by the National Science Foundation of China (No. 11201306 and No. 61572327); the Innovation Program of Shanghai Municipal Education Commission (No. 13YZ065); the Natural Science Foundation of Hunan, China (Grant No. 2018JJ2461); the Wannan Medical College Young Scientists Research Fund (No. WK201102); and the Anhui Key Laboratory of Polysaccharide Drug Engineering Foundation (No. XZ201176). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
            Categories
            Research Article
            Biology and Life Sciences
            Biochemistry
            Glycobiology
            Polysaccharides
            Biology and Life Sciences
            Physiology
            Immune Physiology
            Spleen
            Medicine and Health Sciences
            Physiology
            Immune Physiology
            Spleen
            Biology and Life Sciences
            Cell Biology
            Cell Processes
            Phagocytosis
            Biology and Life Sciences
            Cell Biology
            Cellular Types
            Animal Cells
            Blood Cells
            White Blood Cells
            T Cells
            Biology and Life Sciences
            Cell Biology
            Cellular Types
            Animal Cells
            Immune Cells
            White Blood Cells
            T Cells
            Biology and Life Sciences
            Immunology
            Immune Cells
            White Blood Cells
            T Cells
            Medicine and Health Sciences
            Immunology
            Immune Cells
            White Blood Cells
            T Cells
            Biology and Life Sciences
            Cell Biology
            Cellular Types
            Animal Cells
            Blood Cells
            White Blood Cells
            Macrophages
            Biology and Life Sciences
            Cell Biology
            Cellular Types
            Animal Cells
            Immune Cells
            White Blood Cells
            Macrophages
            Biology and Life Sciences
            Immunology
            Immune Cells
            White Blood Cells
            Macrophages
            Medicine and Health Sciences
            Immunology
            Immune Cells
            White Blood Cells
            Macrophages
            Physical Sciences
            Chemistry
            Chemical Compounds
            Organic Compounds
            Carbohydrates
            Monosaccharides
            Physical Sciences
            Chemistry
            Organic Chemistry
            Organic Compounds
            Carbohydrates
            Monosaccharides
            Biology and Life Sciences
            Immunology
            Immune Suppression
            Medicine and Health Sciences
            Immunology
            Immune Suppression
            Medicine and Health Sciences
            Diagnostic Medicine
            Signs and Symptoms
            Immune Suppression
            Medicine and Health Sciences
            Pathology and Laboratory Medicine
            Signs and Symptoms
            Immune Suppression
            Biology and Life Sciences
            Immunology
            Immune System
            Thymus
            Medicine and Health Sciences
            Immunology
            Immune System
            Thymus
            Custom metadata
            All the data used in this study is included in Tables and Figures.

            Uncategorized

            Comments

            Comment on this article