Blog
About

23
views
0
recommends
+1 Recommend
1 collections
    2
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Rapid screening and identification of sesquiterpene lactones in Kudiezi injection based on high-performance liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Sesquiterpene lactones are considered as the major active compounds in Kudiezi injection in virtue of their special structures and activities. Herein, an analytical method was developed for rapid screening and identification of sesquiterpene lactones in Kudiezi injection using high-performance liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry (HPLC-LTQ-Orbitrap) in negative ion mode. First, two sesquiterpene lactone reference standards were analyzed to obtain their characteristic ESI-MS/MS fragmentation patterns. Second, based on extracted ion chromatography (EIC) data-mining method and characteristic fragmentation pathways analysis, sesquiterpene lactones in Kudiezi injection were rapidly screened and identified. Finally, an important parameter Clog P was adopted to discriminate the isomers of sesquiterpene lactones. As a result, 50 ses-quiterpene lactones were characterized, including 9 sesquiterpene lactone agly cones, 39 sesquiterpene lactone glycosides, and 2 amino acid-sesquiterpene lactone conjugates. Among them, 13 compounds were tentatively identified as new compounds. The results demonstrated that the established method would be a rapid, effective analytical tool for screening and identification of ses-quiterpene lactones in the complex system of natural medicines.

          Related collections

          Author and article information

          Journal
          CJNM
          Chinese Journal of Natural Medicines
          Elsevier
          1875-5364
          20 February 2018
          : 16
          : 2
          : 150-160
          Affiliations
          1School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100029, China
          2Beijing Research Institution of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
          3Library of Beijing University of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
          Author notes
          *Corresponding authors: ZHANG Jia-Yu, Tel: 86-10-64287540, E-mail: zhangjiayu0615@ 123456163.com ; LU Jian-Qiu, Tel: 86-10-64287502, E-mail: lujq@ 123456vip.sina.com

          ΔThese two authors contributed equally to this work.

          These authors have no conflict of interest to declare.

          Article
          S1875-5364(18)30042-6
          10.1016/S1875-5364(18)30042-6
          Copyright © 2018 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
          Funding
          Funded by: Beijing Nova Program
          Award ID: Z171100001117029
          Funded by: National Natural Science Foundation of China
          Award ID: 81503244
          This work was supported by Beijing Nova Program (No. Z171100001117029) and the National Natural Science Foundation of China (No. 81503244).

          Comments

          Comment on this article