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Four cDNAs encoding lipoprotein receptors from shrimp (Pandalopsis japonica): structural characterization and expression analysis during maturation.

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      Abstract

      As in all other oviparous animals, lipoprotein receptors play a critical role in lipid metabolism and reproduction in decapod crustaceans. Four full-length cDNAs encoding lipoprotein receptors (Paj-VgR, Paj-LpR1, Paj-LpR2A, and Paj-LpR2B) were identified from Pandalopsis japonica through a combination of EST screening and PCR-based cloning. Paj-LpR1 appears to be the first crustacean ortholog of insect lipophorin receptors, and its two paralogs, Paj-LpR2A and Paj-LpR2B, exhibited similar structural characteristics. Several transcriptional isoforms were also identified for all three Paj-LpRs. Each expression pattern was unique, suggesting different physiological roles for these proteins. Paj-VgR is an ortholog of vitellogenin (Vg) receptors from other decapod crustaceans. A phylogenetic analysis of lipoproteins and their receptors suggested that the nomenclature of Vgs from decapod crustaceans may need to be changed. A PCR-based transcriptional analysis showed that Paj-VgR and Paj-LpR2B are expressed almost exclusively in the ovary, whereas Paj-LpR1 and Paj-LpR2A are expressed in multiple tissues. The various transcriptional isoforms of the three Paj-LpRs exhibited unique tissue distribution profiles. A transcriptional analysis of each receptor using tissues with different GSI values showed that the change in transcription of Paj-VgRs, Paj-LpR2A and Paj-LpR1 was not as significant as that of Vgs during maturation. However, the transcriptional levels of Paj-LpR2B decreased in ovary at maturation, suggesting that their transcriptional regulation is involved in reproduction.

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      Affiliations
      [1 ] Interdisciplinary program of Biomedical Engineering, Pukyong National University, Busan, 608-737, South Korea.
      [2 ] Fisheries Resources Research Division, National Fisheries Research and Development Institute, Busan, 619-902, South Korea.
      [3 ] Gyeongsangnam-do Fisheries Resources Research Institute, South Korea.
      [4 ] POSTECH Ocean Science and Technology Institute, Pohang University of Science and Technology (POSTECH), Pohang 790-784, South Korea.
      [5 ] Department of Marine Biology, Pukyong National University, Busan 608-737, South Korea.
      [6 ] Department of Marine Biology, Pukyong National University, Busan 608-737, South Korea; Interdisciplinary program of Biomedical Engineering, Pukyong National University, Busan, 608-737, South Korea. Electronic address: kimhw@pknu.ac.kr.
      Journal
      Comp. Biochem. Physiol. B, Biochem. Mol. Biol.
      Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
      1879-1107
      1096-4959
      Mar 2014
      : 169
      24389120
      S1096-4959(13)00212-1
      10.1016/j.cbpb.2013.12.005
      Copyright © 2013 Elsevier Inc. All rights reserved.

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