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      Identificación de Salmonella Enteritidis y Salmonella Typhimurium en cuyes mediante la técnica de PCR múltiple Translated title: Identification of Salmonella Enteritidis and Salmonella Typhimurium in Guinea pigs by the multiplex PCR

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          Abstract

          El objetivo del presente estudio fue identificar mediante PCR múltiple la posible existencia de los serovares Salmonella Typhimurium y Enteritidis en 25 cepas de Salmonella spp previamente aisladas de cuyes e identificadas por sus características metabólicas. Mediante el análisis molecular se identificaron todas las cepas como Salmonella Typhimurium, evidenciando la amplificación de los cebadores específicos para los genes invAy fliC pertenecientes al género Salmonella y Salmonella Typhimurium, respectivamente. El presente estudio permitió establecer una metodología rápida para la identificación molecular de Salmonella Typhimurium y Enteritidis aislados de cuyes.

          Translated abstract

          The aim of this study was to identify by mutiplex PCR-based assays the possible presence of serovars Salmonella Typhimurium and Enteritidis in 25 strains of Samonella spp that were previously isolated from guinea pigs and identified by their metabolic characteristics. The molecular analysis identified all 25 strains as Salmonella Typhimurium, evidencing the primer amplification of genes invA and fliC of Salmonella spp and Salmonella Typhimurium, respectively. This study established a rapid methodology for molecular identification of Salmonella Typhimurium and Enteritidis isolated from guinea pigs

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          Identification of genes associated with survival of Salmonella enterica serovar Enteritidis in chicken egg albumen.

          Salmonella enterica consists of over 2,000 serovars that are major causes of morbidity and mortality associated with contaminated food. Despite similarities among serovars of Salmonella enterica, many demonstrate unique host specificities, epidemiological characteristics, and clinical manifestations. One of the unique epidemiological characteristics of the serovar Enteritidis is that it is the only bacterium routinely transmitted to humans through intact chicken eggs. Therefore, Salmonella enterica serovar Enteritidis must be able to persist inside chicken eggs to be transmitted to humans, and its survival in egg is important for its transmission to the human population. The ability of Salmonella enterica serovar Enteritidis to survive in and transmit through eggs may have contributed to its drastically increased prevalence in the 1980s and 1990s. In the present study, using transposon-mediated mutagenesis, we have identified genes important for the association of Salmonella enterica serovar Enteritidis with chicken eggs. Our results indicate that genes involved in cell wall structural and functional integrity, and nucleic acid and amino acid metabolism are important for Salmonella enterica serovar Enteritidis to persist in egg albumen. Two regions unique to Salmonella enterica serovar Enteritidis were also identified, one of which enhanced the survival of a Salmonella enterica serovar Typhimurium isolate in egg albumen. The implication of our results to the serovar specificity of Salmonella enterica is also explored in the present study.
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            Antigenic Formulae of the Salmonella Serovars

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              Identification by a multiplex PCR-based assay of Salmonella typhimurium and Salmonella enteritidis strains from environmental swabs of poultry houses.

              A multiplex-PCR-based assay (m-PCR) was developed for the detection of Salmonella and for the identification of the two serotypes Enteritidis and Typhimurium. Three sets of primers selected from different genomic sequences amplified a 429 bp fragment specific for the genus Salmonella within a randomly cloned sequence, a 559 bp target specific for Salmonella Typhimurium within the fliC gene and a 312 bp fragment specific for Salmonella Enteritidis within the sefA gene. The m-PCR-based assay was used for detecting Salmonella from 1078 environmental swabs of poultry houses. Prior to PCR, these swabs were pre-enriched in phosphate-buffered peptone water for 18-20 h and then sub-cultured on a Modified Semi-solid Rappaport Vassiliadis medium (MSRV) for 18-20 h. The m-PCR combined with MSRV had a better sensitivity (95%) than the bacteriological method (92.5%). The MSRV-m-PCR assay and the bacteriological method had an agreement rate of 95.6%.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rivep
                Revista de Investigaciones Veterinarias del Perú
                Rev. investig. vet. Perú
                Universidad Nacional Mayor de San Marcos. Facultad de Medicina Veterinaria (Lima, , Peru )
                1609-9117
                April 2017
                : 28
                : 2
                : 411-417
                Affiliations
                [02] orgnameUniversidad Nacional de San Cristóbal de Huamanga orgdiv1Laboratorio de Patología Clínica, Histología y Embriología
                [01] Lima orgnameUniversidad Nacional Mayor de San Marcos orgdiv1Facultad de Medicina Veterinaria Perú
                Article
                S1609-91172017000200020
                10.15381/rivep.v28i2.13074
                75e10f36-78b0-44dd-9968-d42f628758e1

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 27 April 2016
                : 31 January 2017
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 23, Pages: 7
                Product

                SciELO Peru


                guinea pigs,Salmonella Typhimurium,Salmonella Enteritidis,multiplex PCR,cuyes,PCR múltiple

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