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      Effect of Na-Hyaluronan on Stromal and Endothelial Healing in Experimental Corneal Alkali Wounds

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          The healing of stroma and endothelium after a standardized corneal alkali wound was evaluated in sodium-hyaluronan (Na-HA)-treated eyes. Alkali wounds were produced in one eye of each rabbit by applying a 5.5-mm round filter paper soaked in 1  N NaOH onto the central cornea for 60 s. Eyes were then treated with either 1% Na-HA (the treatment group) or phosphate-buffered saline (the control group) 4 times per day for 3 weeks. Endothelial wound morphometry was performed after alizarin red and trypan blue staining, while stromal healing was assessed by counting polymorphonuclear leukocytes (PMNs) and keratocytes in the central and marginal wound areas. During the early healing period the stroma treated with Na-HA had less PMNs than that of the control group. The size of the endothelial defect area measured 5 days after injury was significantly smaller in the Na-HA group than in the control corneas. The present findings indicate that topically applied 1% Na-HA affects stromal and endothelial healing during the early repair process after corneal alkali wounds.

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          Effect of Topical Na-Hyaluronan on Hemidesmosome Formation in n-Heptanol-Induced Corneal Injury

          The present study investigated the effect of Na-hyaluronan (Na-HA) on the hemidesmosome morphogenesis in n-heptanol-induced corneal wounds. Central epithelial wounds were induced in the rabbit cornea by applying a 5.5-mm round filter paper, which was soaked in n-heptanol, for 60 s. 1% Na-HA in phosphate-buffered saline (PBS) or PBS alone were topically administered to the wounded cornea 4 times daily for up to 7 days. Epithelial healing rates during the first 2 days were not altered by Na-HA. The number of hemidesmosomes in the basement membrane of the central cornea was significantly increased in both 3- and 7-day groups after treatment with 1% Na-HA. The results suggest that topically applied 1% Na-HA may enhance the formation of hemidesmosomes during the early healing phase in n-heptanol-induced corneal wounds.
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            The cornea surface and wound healing


              Author and article information

              Ophthalmic Res
              Ophthalmic Research
              S. Karger AG
              December 1999
              30 September 1999
              : 31
              : 6
              : 432-439
              Departments of aOphthalmology and bPharmacology, Medical College, and cDivision of Morphology, Medical Research Center, Ewha Women’s University, Seoul, Korea
              55568 Ophthalmic Res 1999;31:432–439
              © 1999 S. Karger AG, Basel

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              Page count
              Figures: 3, Tables: 1, References: 36, Pages: 8
              Original Paper


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