Evolving Models of Pavlovian Conditioning: Cerebellar Cortical Dynamics in Awake Behaving Mice

Summary

Three decades of electrophysiological research on cerebellar cortical activity underlying Pavlovian conditioning have expanded our understanding of motor learning in the brain. Purkinje cell simple spike suppression is considered to be crucial in the expression of conditional blink responses (CRs). However, trial-by-trial quantification of this link in awake behaving animals is lacking, and current hypotheses regarding the underlying plasticity mechanisms have diverged from the classical parallel fiber one to the Purkinje cell synapse LTD hypothesis. Here, we establish that acquired simple spike suppression, acquired conditioned stimulus (CS)-related complex spike responses, and molecular layer interneuron (MLI) activity predict the expression of CRs on a trial-by-trial basis using awake behaving mice. Additionally, we show that two independent transgenic mouse mutants with impaired MLI function exhibit motor learning deficits. Our findings suggest multiple cerebellar cortical plasticity mechanisms underlying simple spike suppression, and they implicate the broader involvement of the olivocerebellar module within the interstimulus interval.

Graphical Abstract

Highlights

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  Simple spike suppression correlates trial by trial to conditioned eyelid behavior

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  Conditioned stimulus-related complex spikes relate to simple spikes and behavior

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  Molecular layer interneuron (MLI) modulation correlates to behavior

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  Transgenic deficits in MLI input result in partially impaired eyeblink conditioning

Abstract

Purkinje cell simple spike suppression is a central driving mechanism in cerebellar conditioning. Here, ten Brinke et al. show how simple spike suppression, conditioned stimulus-related complex spikes, and molecular layer interneuron (MLI) activity correlate to conditioned eyelid behavior. Moreover, transgenic impairment of MLI input results in deficits in conditioned behavior.