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      Antimicrobial Activity of the Extract of Stem Bark of Diplotropis Ferruginea Benth

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          Abstract

          This is the first report about the antibacterial activity of Diplotropis ferruginea Benth. In this study, the ethanol extract of D. ferruginea was tested for its antimicrobial activity against strains gram-positive and gram-negative. In order to determine the minimal inhibitory concentration, assays were carried out by micro dilution method. The extract was screened for antimicrobial activity, and it showed antibacterial activity against Escherichia coli and Pseudomonas aeruginosa.

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          Most cited references24

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          A new method for determining the minimum inhibitory concentration of essential oils.

          A new microdilution method has been developed for determining the minimum inhibitory concentration (MIC) of oil-based compounds. The redox dye resazurin was used to determine the MIC of a sample of the essential oil of Melaleuca alternifolia (tea tree) for a range of Gram-positive and -negative bacteria. Use of 0.15% (w/v) agar as a stabilizer overcame the problem of adequate contact between the oil and the test bacteria and obviated the need to employ a chemical emulsifier. A rapid version of the assay was also developed for use as a screening method. A comparison of visual and photometric reading of the microtitre plates showed that results could be assessed without instrumentation; moreover, if the rapid assay format was used, rigorous asepsis was not necessary. Accuracy of the resazurin method was confirmed by plate counting from microwells and MIC values were compared with results obtained using an agar dilution assay. The MIC results obtained by the resazurin method were slightly lower than those obtained by agar dilution.
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            Antibacterial Activity of Lantana camara Linn and Lantana montevidensis Brig Extracts from Cariri-Ceará, Brazil

            The use of medicinal plants with therapeutics properties represents a secular tradition in different cultures, mainly in underdeveloped countries. Lantana camara Linn and Lantana montevidensis Briq (Verbenaceae) found in tropical and subtropical areas around the world are popularly known as “camará” or “chumbinho.” In popular medicines, both plants are used as antipyretic and carminative and in the treatment of respiratory system infections. In this study, the antibacterial activity of the ethanolic extracts of L. camara and L. montevidensis leaves and roots against gram-positive and gram-negative strains standard and multi-resistant bacteria isolated from clinical material are presented. In order to determine the minimal inhibitory concentration (MIC), the microdilution method was used. The extracts demonstrated antibacterial activity against all tested bacteria, but the L. montevidensis fresh leaves extract present the best result against P. aeruginosa (MIC 8 μg/mL) and against multi-resistant E. coli (Ec 27) (MIC 16 μg/mL). These results drive new researches with both species in order to isolate the constituents responsible for the activity.
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              Flowering plants of the world

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                Author and article information

                Journal
                J Young Pharm
                JYPharm
                Journal of Young Pharmacists : JYP
                Medknow Publications & Media Pvt Ltd (India )
                0975-1483
                0975-1505
                Oct-Dec 2011
                : 3
                : 4
                : 284-286
                Affiliations
                [1] Department of Physiology and Pharmacology, Rua Cel. Nunes de Melo, Fortaleza, Brazil
                [1 ] Department of Medicine, Federal University of Vale do São Francisco, Brazil
                [2 ] Department of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, Brazil
                [3 ] Department of Pharmaceutical Sciences, Federal University of Piaui, Teresina, Brazil
                Author notes
                Address for correspondence: Dr. Gilberto Santos Cerqueira; E-mail: giufarmacia@ 123456hotmail.com
                Article
                JYPharm-3-284
                10.4103/0975-1483.90237
                3249740
                22224034
                7673d099-baf5-44d3-8ad3-5b97c784064d
                Copyright: © Journal of Young Pharmacists

                This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Categories
                Pharmacognosy

                Pharmacology & Pharmaceutical medicine
                escherichia coli,fabaceae,antimicrobial activity,diplotropis ferruginea,pseudomonas aeruginosa

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