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      Aqueous two-phase system (ATPS): an overview and advances in its applications

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          Abstract

          Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained an interest because of great potential for the extraction, separation, purification and enrichment of proteins, membranes, viruses, enzymes, nucleic acids and other biomolecules both in industry and academia. Although, the partition behavior involved in the method is complex and difficult to predict. Current research shows that it has also been successfully used in the detection of veterinary drug residues in food, separation of precious metals, sewage treatment and a variety of other purposes. The ATPS is able to give high recovery yield and is easily to scale up. It is also very economic and environment friendly method. The aim of this review is to overview the basics of ATPS, optimization and its applications.

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          Remediation of dyes in textile effluent: a critical review on current treatment technologies with a proposed alternative.

          The control of water pollution has become of increasing importance in recent years. The release of dyes into the environment constitutes only a small proportion of water pollution, but dyes are visible in small quantities due to their brilliance. Tightening government legislation is forcing textile industries to treat their waste effluent to an increasingly high standard. Currently, removal of dyes from effluents is by physio-chemical means. Such methods are often very costly and although the dyes are removed, accumulation of concentrated sludge creates a disposal problem. There is a need to find alternative treatments that are effective in removing dyes from large volumes of effluents and are low in cost, such as biological or combination systems. This article reviews the current available technologies and suggests an effective, cheaper alternative for dye removal and decolourisation applicable on large scale.
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            Design and Analysis of Experiments

            (2001)
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              Phase separation of integral membrane proteins in Triton X-114 solution.

              C Bordier (1981)
              A solution of the nonionic detergent Triton X-114 is homogeneous at 0 degrees C but separates in an aqueous phase and a detergent phase above 20 degrees C. The extent of this detergent phase separation increases with the temperature and is sensitive to the presence of other surfactants. The partition of proteins during phase separation in solutions of Triton X-114 is investigated. Hydrophilic proteins are found exclusively in the aqueous phase, and integral membrane proteins with an amphiphilic nature are recovered in the detergent phase. Triton X-114 is used to solubilize membranes and whole cells, and the soluble material is submitted to phase separation. Integral membrane proteins can thus be separated from hydrophilic proteins and identified as such in crude membrane or cellular detergent extracts.
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                Author and article information

                Contributors
                dr.mujahidiqbal@gmail.com
                tyf@mail.hzau.edu.cn
                xieshuyu@mail.hzau.edu.cn
                yufeizhu@webmail.hzau.edu.cn
                chendongmei@mail.hzau.edu.cn
                wangxu@mail.hzau.edu.cn
                huanglingli@mail.hzau.edu.cn
                pengdapeng@mail.hzau.edu.cn
                dr.adeel86@yahoo.com
                vetwaince@gmail.com
                hih_dvm@yahoo.com
                1901962847@qq.com
                +86 27 87287186 , yuan5802@mail.hzau.edu.cn
                Journal
                Biol Proced Online
                Biol Proced Online
                Biological Procedures Online
                BioMed Central (London )
                1480-9222
                28 October 2016
                28 October 2016
                2016
                : 18
                : 18
                Affiliations
                [1 ]National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan, Hubei 430070 China
                [2 ]MAO Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, Hubei 430070 China
                Article
                48
                10.1186/s12575-016-0048-8
                5084470
                27807400
                767defdc-a012-4212-a4c8-abab39b6da85
                © The Author(s). 2016

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                History
                : 2 July 2016
                : 26 September 2016
                Funding
                Funded by: FundRef http://dx.doi.org/http://dx.doi.org/10.13039/501100004573, Ministry of Agriculture of the People's Republic of China;
                Award ID: 2011BAK10B07-5
                Funded by: Risk Assessment of Quality and Safety of Livestock and Poultry Products
                Award ID: GJFP2016008
                Funded by: Huazhong Agricultural University (CN), Independent Technology Innovation Fund
                Award ID: 2662015PY021
                Categories
                Review
                Custom metadata
                © The Author(s) 2016

                Life sciences
                aqueous two-phase system (atps),biomolecule separation,solvent extraction,veterinary drug residues

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