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      IS1R-mediated plasticity of IncL/M plasmids leads to the insertion of bla OXA-48 into the Escherichia coli Chromosome.

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          Abstract

          The OXA-48 carbapenemase is mainly encoded by ∼ 62-kb IncL/M plasmids. However, chromosome-mediated genes have been observed in Escherichia coli isolates. In this work, we investigated the genetic environment of OXA-48 in members of the family Enterobacteriaceae (n = 22) to understand how the OXA-48-encoding gene is transferred into the E. coli chromosome. The OXA-48-encoding gene was located within intact Tn1999.2 transposons in the ∼ 62-kb plasmids or within a truncated variant of Tn1999.2 for the OXA-48-encoding genes located in the chromosomes of E. coli bacteria. The analysis of the Tn1999.2 genetic environment revealed an inverted orientation of the transposon in five ∼ 62-kb plasmids (5/14 [35%]) and in all chromosome inserts (n = 8). The sequencing of pRA35 plasmid showed that this orientation of Tn1999.2 and the acquisition of an IS1R insertion sequence generated a 21.9-kb IS1R-based composite transposon encoding OXA-48 and designated Tn6237. The sequencing of a chromosomal insert encoding OXA-48 also revealed this new transposon in the E. coli chromosome. PCR mapping showed the presence of this element in all strains harboring an OXA-48-encoding chromosomal insert. However, different insertion sites of this transposon were observed in the E. coli chromosome. Overall, these findings indicate a plasticity of the OXA-48 genetic environment mediated by IS1R insertion sequences. The insertion sequences can induce the transfer of the OXA-encoding gene into E. coli chromosomes and thereby promote its persistence and expression at low levels.

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          Author and article information

          Journal
          Antimicrob. Agents Chemother.
          Antimicrobial agents and chemotherapy
          1098-6596
          0066-4804
          Jul 2014
          : 58
          : 7
          Affiliations
          [1 ] Clermont Université, UMR 1071 INSERM/Université d'Auvergne, Clermont-Ferrand, France INRA, USC 2018, Clermont-Ferrand, France Health and Environment Microbiology Laboratory, AZM Research Centre of Biotechnology, Doctoral School of Sciences and Technology, and Faculty of Public Health, Lebanese University, Tripoli, Lebanon Centre Hospitalier Universitaire, Clermont-Ferrand, France.
          [2 ] Clermont Université, UMR 1071 INSERM/Université d'Auvergne, Clermont-Ferrand, France INRA, USC 2018, Clermont-Ferrand, France Centre Hospitalier Universitaire, Clermont-Ferrand, France.
          [3 ] Clermont Université, UMR 1071 INSERM/Université d'Auvergne, Clermont-Ferrand, France INRA, USC 2018, Clermont-Ferrand, France.
          [4 ] Health and Environment Microbiology Laboratory, AZM Research Centre of Biotechnology, Doctoral School of Sciences and Technology, and Faculty of Public Health, Lebanese University, Tripoli, Lebanon.
          [5 ] Clermont Université, UMR 1071 INSERM/Université d'Auvergne, Clermont-Ferrand, France INRA, USC 2018, Clermont-Ferrand, France Centre Hospitalier Universitaire, Clermont-Ferrand, France rbonnet@chu-clermontferrand.fr.
          Article
          AAC.02669-14
          10.1128/AAC.02669-14
          4068556
          24752261
          76a2d90d-938f-45b4-9497-ac8da596ae39
          Copyright © 2014, American Society for Microbiology. All Rights Reserved.
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