2
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Maternal supplementation with phytogenic additives influenced the faecal microbiota and reproductive potential in sows

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Sows undergo physiological stress during gestation and lactation, potentially leading to enteric dysbiosis and reduced reproductive potential. Phytogenic additives (PFs) may improve performance via their antioxidant, anti-inflammatory and antimicrobial properties. This study determined whether the provision of a gestation/lactation diet containing PAs would alter the gastrointestinal microbiota of sows and their piglets, and improve performance. Sows received a commercial diet throughout gestation and lactation (CTR; n = 64), a commercial diet throughout gestation and a diet containing PAs in lactation (CTR-PA; n = 63) or a commercial diet containing PAs in gestation and lactation (PA; n = 90). Sows were weighed and backfat recorded after mating and at entry and exit from the farrowing house and piglets were weighed on days 1 and 21 of life. Faecal samples collected from sows at farrowing house entry and piglets at 21 and 35 d were subjected to 16 S rRNA gene amplicon analysis. The addition of PAs to sow diets resulted in more piglets born ( P = 0.03), however, it did not improve the number of liveborn piglets ( P = 0.14). There were no differences in sow weight, P2 backfat depth or lactation feed intake observed. PAs had no effect on piglet weight or survival to weaning but did alter the faecal microbiota of sows, and this change was observed in piglets at 21 and 35 d. PA supplementation to sows has the potential to increase litter size, while also potentially influencing gastrointestinal tract health of the sow and piglets reared.

          Supplementary Information

          The online version contains supplementary material available at 10.1186/s13568-021-01268-8.

          Related collections

          Most cited references33

          • Record: found
          • Abstract: not found
          • Article: not found

          Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2

            Bookmark
            • Record: found
            • Abstract: found
            • Article: found
            Is Open Access

            VSEARCH: a versatile open source tool for metagenomics

            Background VSEARCH is an open source and free of charge multithreaded 64-bit tool for processing and preparing metagenomics, genomics and population genomics nucleotide sequence data. It is designed as an alternative to the widely used USEARCH tool (Edgar, 2010) for which the source code is not publicly available, algorithm details are only rudimentarily described, and only a memory-confined 32-bit version is freely available for academic use. Methods When searching nucleotide sequences, VSEARCH uses a fast heuristic based on words shared by the query and target sequences in order to quickly identify similar sequences, a similar strategy is probably used in USEARCH. VSEARCH then performs optimal global sequence alignment of the query against potential target sequences, using full dynamic programming instead of the seed-and-extend heuristic used by USEARCH. Pairwise alignments are computed in parallel using vectorisation and multiple threads. Results VSEARCH includes most commands for analysing nucleotide sequences available in USEARCH version 7 and several of those available in USEARCH version 8, including searching (exact or based on global alignment), clustering by similarity (using length pre-sorting, abundance pre-sorting or a user-defined order), chimera detection (reference-based or de novo), dereplication (full length or prefix), pairwise alignment, reverse complementation, sorting, and subsampling. VSEARCH also includes commands for FASTQ file processing, i.e., format detection, filtering, read quality statistics, and merging of paired reads. Furthermore, VSEARCH extends functionality with several new commands and improvements, including shuffling, rereplication, masking of low-complexity sequences with the well-known DUST algorithm, a choice among different similarity definitions, and FASTQ file format conversion. VSEARCH is here shown to be more accurate than USEARCH when performing searching, clustering, chimera detection and subsampling, while on a par with USEARCH for paired-ends read merging. VSEARCH is slower than USEARCH when performing clustering and chimera detection, but significantly faster when performing paired-end reads merging and dereplication. VSEARCH is available at https://github.com/torognes/vsearch under either the BSD 2-clause license or the GNU General Public License version 3.0. Discussion VSEARCH has been shown to be a fast, accurate and full-fledged alternative to USEARCH. A free and open-source versatile tool for sequence analysis is now available to the metagenomics community.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB

              A 16S rRNA gene database ( http://greengenes.lbl.gov ) addresses limitations of public repositories by providing chimera screening, standard alignment, and taxonomic classification using multiple published taxonomies. It was found that there is incongruent taxonomic nomenclature among curators even at the phylum level. Putative chimeras were identified in 3% of environmental sequences and in 0.2% of records derived from isolates. Environmental sequences were classified into 100 phylum-level lineages in the Archaea and Bacteria .
                Bookmark

                Author and article information

                Contributors
                tanya.nowland@adelaide.edu.au
                Journal
                AMB Express
                AMB Express
                AMB Express
                Springer Berlin Heidelberg (Berlin/Heidelberg )
                2191-0855
                15 July 2021
                15 July 2021
                2021
                : 11
                : 107
                Affiliations
                [1 ]GRID grid.1010.0, ISNI 0000 0004 1936 7304, School of Animal and Veterinary Sciences, , The University of Adelaide, ; 5371 Roseworthy, SA Australia
                [2 ]GRID grid.1023.0, ISNI 0000 0001 2193 0854, Institute for Future Farming Systems, , Central Queensland University, ; 4701 Rockhampton, QLD Australia
                [3 ]GRID grid.464686.e, ISNI 0000 0001 1520 1671, Food Sciences, , South Australian Research and Development Institute, ; SA 5064 Urrbrae, Australia
                [4 ]BIOMIN Pte. Ltd, 159741 Singapore, Singapore
                [5 ]SunPork Group, QLD 4009 Eagle Farm, Australia
                Author information
                http://orcid.org/0000-0001-8812-5948
                Article
                1268
                10.1186/s13568-021-01268-8
                8282833
                34264424
                76a8d1d5-5eff-45a4-ae47-5a733b14fce6
                © The Author(s) 2021

                Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 1 July 2021
                : 6 July 2021
                Funding
                Funded by: Biomin (AT)
                Award ID: Biomin (AT)
                Award Recipient :
                Categories
                Original Article
                Custom metadata
                © The Author(s) 2021

                Biotechnology
                bacteria,gut health,production,pig,pfa
                Biotechnology
                bacteria, gut health, production, pig, pfa

                Comments

                Comment on this article