Bioprocessing of tea oil fruit hull with acetic acid organosolv pretreatment in combination with alkaline H2O2

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Abstract

Background

As a natural renewable biomass, the tea oil fruit hull (TOFH) mainly consists of lignocellulose, together with some bioactive substances. Our earlier work constructed a two-stage solvent-based process, including one aqueous ethanol organosolv extraction and an atmospheric glycerol organosolv (AGO) pretreatment, for bioprocessing of the TOFH into diverse bioproducts. However, the AGO pretreatment is not as selective as expected in removing the lignin from TOFH, resulting in the limited delignification and simultaneously high cellulose loss.

Results

In this study, acetic acid organosolv (AAO) pretreatment was optimized with experimental design to fractionate the TOFH selectively. Alkaline hydrogen peroxide (AHP) pretreatment was used for further delignification. Results indicate that the AAO–AHP pretreatment had an extremely good selectivity at component fractionation, resulting in 92% delignification and 88% hemicellulose removal, with 87% cellulose retention. The pretreated substrate presented a remarkable enzymatic hydrolysis of 85% for 48 h at a low cellulase loading of 3 FPU/g dry mass. The hydrolyzability was correlated with the composition and structure of substrates by using scanning electron microscopy, confocal laser scanning microscopy, and X-ray diffraction.

Conclusion

The mild AAO–AHP pretreatment is an environmentally benign and advantageous scheme for biorefinery of the agroforestry biomass into value-added bioproducts.

Electronic supplementary material

The online version of this article (doi:10.1186/s13068-017-0777-1) contains supplementary material, which is available to authorized users.

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Most cited references 42

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The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials.

Runcang Sun, Xuefei Cao, Shaolong Sun … (2016)

Lignocellulosic materials are among the most promising alternative energy resources that can be utilized to produce cellulosic ethanol. However, the physical and chemical structure of lignocellulosic materials forms strong native recalcitrance and results in relatively low yield of ethanol from raw lignocellulosic materials. An appropriate pretreatment method is required to overcome this recalcitrance. For decades various pretreatment processes have been developed to improve the digestibility of lignocellulosic biomass. Each pretreatment process has a different specificity on altering the physical and chemical structure of lignocellulosic materials. In this paper, the chemical structure of lignocellulosic biomass and factors likely affect the digestibility of lignocellulosic materials are discussed, and then an overview about the most important pretreatment processes available are provided. In particular, the combined pretreatment strategies are reviewed for improving the enzymatic hydrolysis of lignocellulose and realizing the comprehensive utilization of lignocellulosic materials.

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Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment

Shishir P. S. Chundawat, Bryon S. Donohoe, Leonardo da Costa Sousa … (2011)

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Understanding the structural and chemical changes of plant biomass following steam explosion pretreatment

Thomas Auxenfans, David Crônier, Brigitte Chabbert … (2017)

Background Biorefining of lignocellulosic biomass has become one of the most valuable alternatives for the production of multi-products such as biofuels. Pretreatment is a prerequisite to increase the enzymatic conversion of the recalcitrant lignocellulose. However, there is still considerable debate regarding the key features of biomass impacting the cellulase accessibility. In this study, we evaluate the structural and chemical features of three different representative biomasses (Miscanthus × giganteus, poplar and wheat straw), before and after steam explosion pretreatment at increasing severities, by monitoring chemical analysis, SEM, FTIR and 2D NMR. Results Regardless the biomass type, combined steam explosion pretreatment with dilute sulfuric acid impregnation resulted in significant improvement of the cellulose conversion. Chemical analyses revealed that the pretreatment selectively degraded the hemicellulosic fraction and associated cross-linking ferulic acids. As a result, the pretreated residues contained mostly cellulosic glucose and lignin. In addition, the pretreatment directly affected the cellulose crystallinity but these variations were dependent upon the biomass type. Important chemical modifications also occurred in lignin since the β-O-4′ aryl-ether linkages were found to be homolytically cleaved, followed by some recoupling/recondensation to β-β′ and β-5′ linkages, regardless the biomass type. Finally, 2D NMR analysis of the whole biomass showed that the pretreatment preferentially degraded the syringyl-type lignin fractions in miscanthus and wheat straw while it was not affected in the pretreated poplar samples. Conclusions Our findings provide an enhanced understanding of parameters impacting biomass recalcitrance, which can be easily generalized to both woody and non-woody biomass species. Results indeed suggest that the hemicellulose removal accompanied by the significant reduction in the cross-linking phenolic acids and the redistribution of lignin are strongly correlated with the enzymatic saccharification, by loosening the cell wall structure thus allowing easier cellulase accessibility. By contrast, we have shown that the changes in the syringyl/guaiacyl ratio and the cellulose crystallinity do not seem to be relevant factors in assessing the enzymatic digestibility. Some biomass type-dependent and easily measurable FTIR factors are highly correlated to saccharification.

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Author and article information

Contributors

Song Tang: tangsongfuelbiol@163.com

Rukuan Liu: liurukuan@gmail.com

Fubao Fuelbiol Sun: fubaosun@jiangnan.edu.cn

Chunying Dong: 1219921705@qq.com

Rui Wang: gjyoucha@163.com

Zhongyuan Gao: 490509257@qq.com

Zhanying Zhang: jan.zhang@qut.edu.au

Zhihong Xiao: xzhh1015@163.com

Changzhu Li: lichangzhu2013@aliyun.com

Hui Li: lihuiluoyang@163.com

Journal

Journal ID (nlm-ta): Biotechnol Biofuels

Journal ID (iso-abbrev): Biotechnol Biofuels

Title: Biotechnology for Biofuels

Publisher: BioMed Central (London )

ISSN (Electronic): 1754-6834

Publication date (Electronic): 8 April 2017

Publication date PMC-release: 8 April 2017

Publication date Collection: 2017

Volume: 10

Electronic Location Identifier: 86

Affiliations

[1 ]GRID grid.258151.a, Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, , Jiangnan University, ; Wuxi, 214122 China

[2 ]GRID grid.79703.3a, State Key Laboratory of Pulp and Paper Engineering, , South China University of Technology, ; Guangzhou, 510640 China

[3 ]National Engineering Research Center for Oil-tea Camellia, Hunan Academy of Forestry, Changsha, 410004 China

[4 ]GRID grid.413273.0, Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Ministry of Education, , Zhejiang Sci-Tech University, ; Hangzhou, 310018 China

[5 ]GRID grid.1024.7, Centre for Tropical Crops and Biocommodities, , Queensland University of Technology, ; Brisbane, QLD 4001 Australia

Article

Publisher ID: 777

DOI: 10.1186/s13068-017-0777-1

PMC ID: 5385081

PubMed ID: 28053662

SO-VID: 76c2e15b-74dd-49bf-8ffc-7d71fd933c09

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

History

Date received : 15 February 2017

Date accepted : 5 April 2017

Funding

Funded by: FundRef http://dx.doi.org/10.13039/501100001809, National Natural Science Foundation of China;

Award ID: 21176106

Award ID: 21407046

Award Recipient : Fubao Fuelbiol Sun Hui Li

Funded by: Opening Project of National Engineering Research Center for Oil-tea Camellia

Award ID: 2014CY01

Award Recipient : Fubao Fuelbiol Sun

Funded by: State Key Laboratory of Pulp and Paper Engineering

Award ID: 201513

Award Recipient : Fubao Fuelbiol Sun

Custom metadata

ScienceOpen disciplines: Biotechnology

Keywords: tea oil fruit hull,acetic acid,alkaline h2o2,mild pretreatment,delignification,enzymatic hydrolysis

Data availability:

ScienceOpen disciplines: Biotechnology

Keywords: tea oil fruit hull, acetic acid, alkaline h2o2, mild pretreatment, delignification, enzymatic hydrolysis