+1 Recommend
1 collections
      Are you tired of sifting through news that doesn't interest you?
      Personalize your Karger newsletter today and get only the news that matters to you!

      Sign up

      • Record: found
      • Abstract: found
      • Article: found

      Effect of a Prolyl Hydroxylase Inhibitor on Rabbit Ocular Fibroblasts


      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          We examined the effect of a prolyl hydroxylase inhibitor, ethyl-3,4-dihydroxybenzoate (E-DHB), on collagen production, proliferation, and spreading by cultured rabbit keratocytes and subconjunctival fibroblasts. Proliferating cells were incubated with or without E-DHB, and were examined by phase-contrast microscopy and proliferation assays. In addition, wounds were made in confluent cultures and were allowed to heal with or without exposure to E-DHB. Confluent cells were also observed by transmission electron microscopy after exposure to E-DHB (0.2-0.4 m M). Finally, confluent cells were incubated in the presence or absence of E-DHB, after which the concentration of soluble collagen in the culture medium was determined. E-DHB (0.2-0.4 m M) inhibited the growth of rabbit keratocytes and subconjunctival fibroblasts and decreased collagen production without inducing cytotoxicity. Wounds in the control culture were initially healed by individual migrating cells and later by spreading monolayer sheets and proliferating cells, while E-DHB inhibited cell migration into the wound. Electron microscopy revealed large irregular cisternae of endoplasmic reticulum in E-DHB-treated cells, possibly due to protein accumulation. These findings suggest that collagen production may be closely related to various cellular activities.

          Related collections

          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          11 December 2009
          : 27
          : 6
          : 335-346
          Departments of aOphthalmology and bPathology, Wakayama Medical College, Wakayama, Japan
          267745 Ophthalmic Res 1995;27:335–346
          © 1995 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 12
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Cell culture,Ethyl-3,4-dihydroxybenzoate,Prolyl 4-hydroxylase,Keratocyte,Subconjunctival fibroblast


          Comment on this article