Derivation and characterization of integration-free iPSC line ISRM-UM51 derived from SIX2-positive renal cells isolated from urine of an African male expressing the CYP2D6 *4/*17 variant which confers intermediate drug metabolizing activity.

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Abstract

SIX2-positive renal cells isolated from urine from a 51year old male of African origin bearing the CYP2D6 *4/*17 variant were reprogrammed by nucleofection of a combination of two episomal-based plasmids omitting pathway (TGFβ, MEK and GSK3β) inhibition. The induced pluripotent stem cells (iPSCs) were characterized by immunocytochemistry, embryoid body formation, DNA-fingerprinting and karyotype analysis. Comparative transcriptome analyses with human embryonic stem cell lines H1 and H9 revealed a Pearson correlation of 0.9243 and 0.9619 respectively.

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Journal

Journal ID (iso-abbrev): Stem Cell Res

Title: Stem cell research

Publisher: Elsevier BV

ISSN (Electronic): 1876-7753

ISSN (Print): 1873-5061

Publication date (Electronic): Dec 2017

Volume: 25

Affiliations

[1 ] Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.

[2 ] Institute of Transplantation Diagnostics and Cell Therapeutics, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.

[3 ] Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany. Electronic address: james.adjaye@med.uni-duesseldorf.de.

Article

Publisher Item ID: S1873-5061(17)30209-X

DOI: 10.1016/j.scr.2017.10.004

PubMed ID: 29035842

SO-VID: 772eff09-063c-4edc-8a84-31ec4d064ff5

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Derivation and characterization of integration-free iPSC line ISRM-UM51 derived from SIX2-positive renal cells isolated from urine of an African male expressing the CYP2D6 4/17 variant which confers intermediate drug metabolizing activity.

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