0
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      In vitro Nuclear 3H-Hydrocortisone Binding in the Hypothalamus of the Pig

      ,

      Neuroendocrinology

      S. Karger AG

      3H-hydrocortisone , Binding, Hypothalamic cell nuclei, Pig

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Hypothalamic cell nuclear uptake and binding of <sup>3</sup>H-hydrocortisone(<sup>3</sup>H-HC)-receptor complexes were studied in a cell-free system. Greater net concentrations of radioactivity were found in the 0.4 MKC1 non-extractable fraction (sediment) in the first 20 min of incubation and in the 0.4 MKC1 extractable fraction (supernatant) from 30 to 60 min. Incubation in low ionic strength medium favored localization of radioactivity in the supernatant, whereas high ionic strength promoted greater localization in the sediment. Specific binding in the supernatant was non-saturable, but reached a plateau in the sediment at about 5 × 10<sup>–8</sup> M <sup>3</sup>H-HC. The observed value of K<sub>d</sub> for binding in the nuclear sediment was 4.88 × 10<sup>9</sup> M<sup>–1</sup> and the maximum number of binding sites was 8.8 femtomoles/mg DNA. RNA polymerase activity increased by 59% in the presence of hormone-receptors over that in the presence of free hormone in buffer.

          Related collections

          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1978
          1978
          26 March 2008
          : 26
          : 3
          : 129-140
          Affiliations
          Department of Physiology and Biophysics, University of Oklahoma Health Sciences Center, Oklahoma City, Okla.
          Article
          122824 Neuroendocrinology 1978;26:129–140
          10.1159/000122824
          683465
          © 1978 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 12
          Categories
          Paper

          Comments

          Comment on this article