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      • Record: found
      • Abstract: found
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      In vitro Nuclear 3H-Hydrocortisone Binding in the Hypothalamus of the Pig

      research-article
      Author(s): ,
      Publication date (Electronic):
      Journal: Neuroendocrinology
      Publisher: S. Karger AG
      Keywords: 3H-hydrocortisone , Binding, Hypothalamic cell nuclei, Pig

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          Abstract

          Hypothalamic cell nuclear uptake and binding of <sup>3</sup>H-hydrocortisone(<sup>3</sup>H-HC)-receptor complexes were studied in a cell-free system. Greater net concentrations of radioactivity were found in the 0.4 MKC1 non-extractable fraction (sediment) in the first 20 min of incubation and in the 0.4 MKC1 extractable fraction (supernatant) from 30 to 60 min. Incubation in low ionic strength medium favored localization of radioactivity in the supernatant, whereas high ionic strength promoted greater localization in the sediment. Specific binding in the supernatant was non-saturable, but reached a plateau in the sediment at about 5 × 10<sup>–8</sup> M <sup>3</sup>H-HC. The observed value of K<sub>d</sub> for binding in the nuclear sediment was 4.88 × 10<sup>9</sup> M<sup>–1</sup> and the maximum number of binding sites was 8.8 femtomoles/mg DNA. RNA polymerase activity increased by 59% in the presence of hormone-receptors over that in the presence of free hormone in buffer.

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          Author and article information

          Journal
          Journal ID (publisher-id): NEN
          Journal ID (nlm-ta): Neuroendocrinology
          Journal ID (doi): 10.1159/issn.0028-3835
          Title: Neuroendocrinology
          Publisher: S. Karger AG
          ISSN (Print): 0028-3835
          ISSN (Electronic): 1423-0194
          Publication date Subscription-year: 1978
          Publication date (Print): 1978
          Publication date (Electronic): 26 March 2008
          Volume: 26
          Issue: 3
          Pages: 129-140
          Affiliations
          Department of Physiology and Biophysics, University of Oklahoma Health Sciences Center, Oklahoma City, Okla.
          Article
          Publisher ID: 122824 Other ID: Neuroendocrinology 1978;26:129–140
          DOI: 10.1159/000122824
          PubMed ID: 683465
          SO-VID: 77534a14-ade6-43e8-a079-769a5f12b8c4
          Copyright © © 1978 S. Karger AG, Basel
          License:

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          Date received : 29 August 1977
          Date accepted : 08 December 1977
          Page count
          Pages: 12
          Categories
          Subject: Paper

          ScienceOpen disciplines: Endocrinology & Diabetes,Neurology,Nutrition & Dietetics,Sexual medicine,Internal medicine,Pharmacology & Pharmaceutical medicine
          Keywords: Hypothalamic cell nuclei,Binding, 3H-hydrocortisone,Pig
          Data availability:
          ScienceOpen disciplines: Endocrinology & Diabetes, Neurology, Nutrition & Dietetics, Sexual medicine, Internal medicine, Pharmacology & Pharmaceutical medicine
          Keywords: Hypothalamic cell nuclei, Binding, 3H-hydrocortisone, Pig

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