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      A Reverse Transcription Genome Exponential Amplification Reaction Assay for Visual Detection of Middle East Respiratory Syndrome Coronavirus

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          Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia.

          A previously unknown coronavirus was isolated from the sputum of a 60-year-old man who presented with acute pneumonia and subsequent renal failure with a fatal outcome in Saudi Arabia. The virus (called HCoV-EMC) replicated readily in cell culture, producing cytopathic effects of rounding, detachment, and syncytium formation. The virus represents a novel betacoronavirus species. The closest known relatives are bat coronaviruses HKU4 and HKU5. Here, the clinical data, virus isolation, and molecular identification are presented. The clinical picture was remarkably similar to that of the severe acute respiratory syndrome (SARS) outbreak in 2003 and reminds us that animal coronaviruses can cause severe disease in humans.
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            Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

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              Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections

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                Author and article information

                Contributors
                Journal
                Infectious Diseases and Translational Medicine
                Infect. Dis. Transl. Med.
                Infect. Dis. Transl. Med.
                International Biological and Medical Journals Publishing House Co., Limited (Room E16, 3/f, Yongda Commercial Building, No.97, Bonham Stand (Sheung Wan), HongKong )
                2411-2917
                30 June 2015
                : 1
                : 1
                : 6-8
                Affiliations
                From the Key Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping district, Beijing, 102206, China
                From the Key Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping district, Beijing, 102206, China
                From the Key Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping district, Beijing, 102206, China; Department of Pathophysiology, Guangzhou Medical University, Guangzhou, Guangdong, 510182, China
                From the Key Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping district, Beijing, 102206, China
                From the Key Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping district, Beijing, 102206, China
                Author notes
                Corresponding author: Xuejun Ma, from the Key Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No.155 Changbai Road, Changping district, Beijing, 102206, China; Tel./Fax: +861058900810; Email: maxj@ 123456ivdc.chinacdc.cn
                Article
                10.11979/idtm.201501003
                77554244-3c5c-47d7-a1dc-a1c932a3db70

                This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

                Page count
                Figures: 2, Tables: 1, References: 8, Pages: 3
                Product
                Categories
                Letter: Detection Techniques

                Medicine,Infectious disease & Microbiology
                Medicine, Infectious disease & Microbiology

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