Synthesis and characterization of Gd-DTPA/fucoidan/peptide complex nanoparticle and in vitro magnetic resonance imaging of inflamed endothelial cells.

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Abstract

P-selectin overexpressed on activated endothelial cells and platelets is a new target for treatment of cancers and cardiovascular diseases such as atherosclerosis and thrombosis. In this study, depolymerized low molecular weight fucoidan (LMWF8775) and a thermolysin-hydrolyzed protamine peptide (TPP1880) were prepared. TPP1880 and LMWF8775 were able to form self-assembled complex nanoparticles (CNPs). The formation of TPP1880/LMWF8775 CNPs was characterized by Fourier-transform infrared spectra, circular dichroism spectra and isothermal titration calorimetry. The CNPs selectively targeted PMA-stimulated, inflamed endothelial cells (HUVECs) with high expression of P-selectin. Gd-DTPA MRI contrast agent was successfully loaded in the CNPs with better T1 relaxivity and selectively accumulated in the activated HUVECs with increased MRI intensity and reduced cytotoxicity as compared to free Gd-DTPA. Our results suggest that the TPP1880/LMWF8775 CNPs may have potential in future for early diagnosis of cardiovascular diseases and cancers in which the endothelium is inflamed or activated.

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Journal ID (iso-abbrev): Mater Sci Eng C Mater Biol Appl

Title: Materials science & engineering. C, Materials for biological applications

Publisher: Elsevier BV

ISSN (Electronic): 1873-0191

ISSN (Print): 0928-4931

Publication date (Electronic): Sep 2020

Volume: 114

Affiliations

[1 ] Graduate Institute of Translational Medicine, College of Medicine and Technology, Taipei Medical University, Taipei 11031, Taiwan.

[2 ] Department of Food Science, College of Life Sciences, National Taiwan Ocean University, Keelung 20224, Taiwan.

[3 ] Research Center of Translational Imaging, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan; Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.

[4 ] Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan.

[5 ] Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan; Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.

[6 ] Graduate Institute of Translational Medicine, College of Medicine and Technology, Taipei Medical University, Taipei 11031, Taiwan; Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Sciences and Technology, Taipei Medical University, Taipei, Taiwan.

[7 ] Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan; School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.

[8 ] Department of Food Science, College of Life Sciences, National Taiwan Ocean University, Keelung 20224, Taiwan. Electronic address: tsai5122@gmail.com.

[9 ] Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan; Department of Biochemistry and Molecular Cell Biology, School of Medicine, Taipei Medical University, Taipei 11031, Taiwan; Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan. Electronic address: flmi530326@tmu.edu.tw.

Article

Publisher Item ID: S0928-4931(20)30378-7

DOI: 10.1016/j.msec.2020.111064

PubMed ID: 32994013

SO-VID: 77726e0e-7b76-40bd-94e8-bb1cc3f6706f

History

Keywords: Targeting,Cell penetrating peptide,Drug delivery,Nanoparticles,Imaging,Fucoidan

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Keywords: Targeting, Cell penetrating peptide, Drug delivery, Nanoparticles, Imaging, Fucoidan

Synthesis and characterization of Gd-DTPA/fucoidan/peptide complex nanoparticle and in vitro magnetic resonance imaging of inflamed endothelial cells.

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