0
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Use of Highly Specific Antibodies against 17α-OH-Progesterone in a Simplified Nonchromatographic RIA and in the Simultaneous Determination of Four Sex Hormones in Human Plasma

      Hormone Research in Paediatrics

      S. Karger AG

      Adult normal values, Chromatography, 17α-Hydroxyprogesterone, Androgens, Radioimmunoassay

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          A highly specific antiserum raised against the 3(O-carboxymethyl)oxime of 17α-hydroxyprogesterone (17-OHP) was produced in rabbit and used in the development of a specific radioimmunoassay (RIA) for 17-OHP which included a celite chromatography. Methods allowing the measurement of plasma 17-OHP levels either separately or in combination with that of several plasma androgens (testosterone, ▵<sup>4</sup>-androstenedione and dehydroepiandrosterone) are described. Moreover, RIA measurement of plasma 17-OHP levels with or without chromatographic (celite column) purification gave comparable results (mean ± SD) in 29 normal adult males (118 ± 34 ng/l00 ml) and 35 normal adult females (follicular phase: 46 ± 16 ng/l00 ml; luteal phase: 241 ± 71 ng/100 ml).

          Related collections

          Author and article information

          Journal
          HRE
          10.1159/issn.0018-5051
          Hormone Research in Paediatrics
          S. Karger AG
          0018-5051
          2571-6603
          1976
          1976
          25 November 2008
          : 7
          : 4-5
          : 260-273
          Affiliations
          Unité de Recherches Endocriniennes et Métaboliques chez I’Enfant, Inserm U 34, Lyon
          Article
          178737 Horm Res 1976;7:260–273
          10.1159/000178737
          © 1976 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 14
          Categories
          Paper

          Comments

          Comment on this article