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      Use of Highly Specific Antibodies against 17α-OH-Progesterone in a Simplified Nonchromatographic RIA and in the Simultaneous Determination of Four Sex Hormones in Human Plasma

      Hormone Research in Paediatrics

      S. Karger AG

      Adult normal values, Chromatography, 17α-Hydroxyprogesterone, Androgens, Radioimmunoassay

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          A highly specific antiserum raised against the 3(O-carboxymethyl)oxime of 17α-hydroxyprogesterone (17-OHP) was produced in rabbit and used in the development of a specific radioimmunoassay (RIA) for 17-OHP which included a celite chromatography. Methods allowing the measurement of plasma 17-OHP levels either separately or in combination with that of several plasma androgens (testosterone, ▵<sup>4</sup>-androstenedione and dehydroepiandrosterone) are described. Moreover, RIA measurement of plasma 17-OHP levels with or without chromatographic (celite column) purification gave comparable results (mean ± SD) in 29 normal adult males (118 ± 34 ng/l00 ml) and 35 normal adult females (follicular phase: 46 ± 16 ng/l00 ml; luteal phase: 241 ± 71 ng/100 ml).

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          Author and article information

          Hormone Research in Paediatrics
          S. Karger AG
          25 November 2008
          : 7
          : 4-5
          : 260-273
          Unité de Recherches Endocriniennes et Métaboliques chez I’Enfant, Inserm U 34, Lyon
          178737 Horm Res 1976;7:260–273
          © 1976 S. Karger AG, Basel

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          Pages: 14


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