Use of Highly Specific Antibodies against 17α-OH-Progesterone in a Simplified Nonchromatographic RIA and in the Simultaneous Determination of Four Sex Hormones in Human Plasma

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Abstract

A highly specific antiserum raised against the 3(O-carboxymethyl)oxime of 17α-hydroxyprogesterone (17-OHP) was produced in rabbit and used in the development of a specific radioimmunoassay (RIA) for 17-OHP which included a celite chromatography. Methods allowing the measurement of plasma 17-OHP levels either separately or in combination with that of several plasma androgens (testosterone, ▵<sup>4</sup>-androstenedione and dehydroepiandrosterone) are described. Moreover, RIA measurement of plasma 17-OHP levels with or without chromatographic (celite column) purification gave comparable results (mean ± SD) in 29 normal adult males (118 ± 34 ng/l00 ml) and 35 normal adult females (follicular phase: 46 ± 16 ng/l00 ml; luteal phase: 241 ± 71 ng/100 ml).

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Journal ID (publisher-id): HRE

Journal ID (doi): 10.1159/issn.0018-5051

Title: Hormone Research in Paediatrics

Publisher: S. Karger AG

ISSN (Print): 0018-5051

ISSN (Electronic): 2571-6603

Publication date Subscription-year: 1976

Publication date (Print): 1976

Publication date (Electronic): 25 November 2008

Volume: 7

Issue: 4-5

Pages: 260-273

Affiliations

Unité de Recherches Endocriniennes et Métaboliques chez I’Enfant, Inserm U 34, Lyon

Article

Publisher ID: 178737 Other ID: Horm Res 1976;7:260–273

DOI: 10.1159/000178737

SO-VID: 77b25987-29f7-4e09-97df-1edeeba1342a

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Use of Highly Specific Antibodies against 17α-OH-Progesterone in a Simplified Nonchromatographic RIA and in the Simultaneous Determination of Four Sex Hormones in Human Plasma

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Karger: Endocrinology

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