Deciphering physio-biochemical, yield, and nutritional quality attributes of water-stressed radish (Raphanus sativus L.) plants grown from Zn-Lys primed seeds

Abstract

Water shortage appears to be expedited under the current climate change scenario worldwide. The present work was aimed to investigate the effects of zinc-chelated lysine (Zn-Lys) on germination and yield of water stressed radish plants. The research was comprised of two studies where the effect of Zn-Lys seed priming on germination attributes under PEG-induced water stress was investigated in the first experiment. In the second experiment, growth, physio-biochemical, and yield responses of water-stressed radish plants raised from Zn-Lys primed seeds were recorded. The seeds pre-conditioned with 0, 1.5, 3, 4.5, or 6 mg kg-1 of Zn-Lys was grown in petri-dishes and pots. Priming treatments significantly improved the germination attributes under water stress. Plants raised from primed seeds exhibited significant improvements in plant biomass production, leaf photosynthetic pigments, final root yield, and nutritional quality. Furthermore, the activities of superoxide dismutase (SOD) and peroxidase (POD) were increased, while the melondialdehyde (MDA) content decreased. Root flavonoids, ascorbic acid, carotenoids, protein, carbohydrates, fiber and lysine content were significantly improved due to Zn-Lys seed priming, both under water-stressed and non-stressed conditions. Moreover, plant's mineral nutrients such as K and Ca as well as Mg, Fe, P, and Zn of final harvested roots were also improved due to Zn-Lys seed priming. Overall, for the induction of drought tolerance and nutritional quality, Zn-Lys regimes of 3 and 4.5 mg kg-1 were most effective. It can be inferred that the Zn-Lys priming maintained a potential balance of nutrient uptake and translocation by preventing drought-induced lipid peroxidation of membranes.