For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
17
views
36
references
 Top references
cited by
33
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      412
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Electrical Guidance of Human Stem Cells in the Rat Brain

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Summary

          Limited migration of neural stem cells in adult brain is a roadblock for the use of stem cell therapies to treat brain diseases and injuries. Here, we report a strategy that mobilizes and guides migration of stem cells in the brain in vivo. We developed a safe stimulation paradigm to deliver directional currents in the brain. Tracking cells expressing GFP demonstrated electrical mobilization and guidance of migration of human neural stem cells, even against co-existing intrinsic cues in the rostral migration stream. Transplanted cells were observed at 3 weeks and 4 months after stimulation in areas guided by the stimulation currents, and with indications of differentiation. Electrical stimulation thus may provide a potential approach to facilitate brain stem cell therapies.

          Graphical Abstract

          Highlights

          • •

            Developed a technology and device delivering electric current to the brain in vivo

          • •

            Achieved stable delivery of currents to brain with monitoring and safety concerns

          • •

            Exhibited effective guidance of migration of transplanted human NSCs in live brain

          • •

            Demonstrated enhanced motility, survival, and differentiation of the guided hNSCs

          Abstract

          In this article, Zhao and colleagues report a novel technology delivering directional electric currents which mobilizes and guides human neural stem cells through the brain in vivo, demonstrating an effective and safe approach to facilitate stem cell therapy, with significant implications for a wide range of brain diseases.

          Related collections

          Most cited references36

          • Record: found
          • Abstract: found
          • Article: not found

          Neuronal replacement from endogenous precursors in the adult brain after stroke.

          Andreas Arvidsson, Tove Collin, Deniz Kirik … (2002)
          In the adult brain, new neurons are continuously generated in the subventricular zone and dentate gyrus, but it is unknown whether these neurons can replace those lost following damage or disease. Here we show that stroke, caused by transient middle cerebral artery occlusion in adult rats, leads to a marked increase of cell proliferation in the subventricular zone. Stroke-generated new neurons, as well as neuroblasts probably already formed before the insult, migrate into the severely damaged area of the striatum, where they express markers of developing and mature, striatal medium-sized spiny neurons. Thus, stroke induces differentiation of new neurons into the phenotype of most of the neurons destroyed by the ischemic lesion. Here we show that the adult brain has the capacity for self-repair after insults causing extensive neuronal death. If the new neurons are functional and their formation can be stimulated, a novel therapeutic strategy might be developed for stroke in humans.
          Article 0 comments Cited 609 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Corridors of Migrating Neurons in Human Brain and Their Decline during Infancy

            Nader Sanai, Thuhien Nguyen, Rebecca A. Ihrie … (2011)
            The subventricular zone (SVZ) of many adult non-human mammals generates large numbers of new neurons destined for the olfactory bulb (OB) 1–6 . Along the walls of the lateral ventricles, immature neuronal progeny migrate in tangentially-oriented chains that coalesce into a rostral migratory stream (RMS) connecting the SVZ to the OB. The adult human SVZ, in contrast, contains a hypocellular gap layer separating the ependymal lining from a periventricular ribbon of astrocytes 7 . Some of these SVZ astrocytes can function as neural stem cells in vitro, but their function in vivo remains controversial. An initial report finds few SVZ proliferating cells and rare migrating immature neurons in the RMS of adult humans 7 . In contrast, a subsequent study indicates robust proliferation and migration in the human SVZ and RMS 8,9 . Here, we find that the infant human SVZ and RMS contain an extensive corridor of migrating immature neurons before 18 months of age, but, contrary to previous reports 8 , this germinal activity subsides in older children and is nearly extinct by adulthood. Surprisingly, during this limited window of neurogenesis, not all new neurons in the human SVZ are destined for the OB – we describe a major migratory pathway that targets the prefrontal cortex in humans. Together, these findings reveal robust streams of tangentially migrating immature neurons in human early postnatal SVZ and cortex. These pathways represent potential targets of neurological injuries affecting neonates.
            Article 0 comments Cited 292 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Electrical signals control wound healing through phosphatidylinositol-3-OH kinase-gamma and PTEN.

              Min Zhao, Bing Song, Jin Pu … (2006)
              Wound healing is essential for maintaining the integrity of multicellular organisms. In every species studied, disruption of an epithelial layer instantaneously generates endogenous electric fields, which have been proposed to be important in wound healing. The identity of signalling pathways that guide both cell migration to electric cues and electric-field-induced wound healing have not been elucidated at a genetic level. Here we show that electric fields, of a strength equal to those detected endogenously, direct cell migration during wound healing as a prime directional cue. Manipulation of endogenous wound electric fields affects wound healing in vivo. Electric stimulation triggers activation of Src and inositol-phospholipid signalling, which polarizes in the direction of cell migration. Notably, genetic disruption of phosphatidylinositol-3-OH kinase-gamma (PI(3)Kgamma) decreases electric-field-induced signalling and abolishes directed movements of healing epithelium in response to electric signals. Deletion of the tumour suppressor phosphatase and tensin homolog (PTEN) enhances signalling and electrotactic responses. These data identify genes essential for electrical-signal-induced wound healing and show that PI(3)Kgamma and PTEN control electrotaxis.
              Article 0 comments Cited 274 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Contributors
                Min Zhao
                Journal
                Journal ID (nlm-ta): Stem Cell Reports
                Journal ID (iso-abbrev): Stem Cell Reports
                Title: Stem Cell Reports
                Publisher: Elsevier
                ISSN (Electronic): 2213-6711
                Publication date PMC-release: 29 June 2017
                Publication date Collection: 11 July 2017
                Publication date (Electronic): 29 June 2017
                Volume: 9
                Issue: 1
                Pages: 177-189
                Affiliations
                [1 ]Departments of Dermatology and Ophthalmology, Institute for Regenerative Cures, University of California Davis, 2921 Stockton Boulevard, Sacramento, CA 95817, USA
                [2 ]Department of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, People's Republic of China
                [3 ]Shanghai Institute of Head Trauma, Shanghai 200127, People's Republic of China
                [4 ]Stem Cell Program and Institute for Regenerative Cures, University of California Davis, Sacramento, CA 95817, USA
                [5 ]Aaken Laboratories, Davis, CA 95616, USA
                [6 ]Department of Neurological Surgery, University of California Davis, Davis, CA 95616, USA
                Author notes
                [∗ ]Corresponding author minzhao@ 123456ucdavis.edu
                Article
                Publisher ID: S2213-6711(17)30262-X
                DOI: 10.1016/j.stemcr.2017.05.035
                PMC ID: 5511115
                PubMed ID: 28669601
                SO-VID: 78f080c9-af37-4e31-a0ac-7f26f0b1cdb7
                Copyright © © 2017 The Author(s)
                License:

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

                History
                Date received : 13 December 2016
                Date revision received : 30 May 2017
                Date accepted : 31 May 2017
                Categories
                Subject: Article

                Keywords: neural stem cells,human neural stem cells,neuroblasts,directional cell migration,electric field,electrotaxis,galvanotaxis,electric stimulation,brain in vivo,in vivo migration
                Data availability:
                Keywords: neural stem cells, human neural stem cells, neuroblasts, directional cell migration, electric field, electrotaxis, galvanotaxis, electric stimulation, brain in vivo, in vivo migration

                Comments

                Comment on this article

                scite_

                Similar content412

                See all similar

                Cited by33

                See all cited by

                Most referenced authors924

                See all reference authors