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Abstract
<p class="first" id="d1629333e71">The purpose of this study was to investigate the
transformation of brushite (dicalcium
phosphate dihydrate, DCPD, CaHPO4·2H2O) powders at 36.5°C in DMEM (Dulbecco's Modified
Eagle Medium) solutions. Two sets of brushite powders with different particle shapes
were synthesized to use in the above DMEM study. The first of these brushite powders
was prepared by using a method which consisted of stirring calcite (CaCO3) powders
in a solution of ammonium dihydrogen phosphate (NH4H2PO4) from 6 to 60min at room
temperature. These powders were found to consist of dumbbells of water lily-shaped
crystals. The second one of the brushite powders had the common flat-plate morphology.
Both powders were separately tested in DMEM-immersion experiments. Monetite (DCPA,
CaHPO4) powders were synthesized with a unique water lily morphology by heating the
water lily-shaped brushite crystals at 200°C for 2h. Brushite powders were found to
transform into octacalcium phosphate (OCP, Ca8(HPO4)2(PO4)4·5H2O) upon soaking in
DMEM (Dulbecco's Modified Eagle Medium) solutions at 36.5°C over a period of 24h to
1week. Brushite powders were known to transform into apatite when immersed in synthetic
(simulated) body fluid (SBF) solutions. This study found that DMEM solutions are able
to convert brushite into OCP, instead of apatite.
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