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      Optimization of urease production by Bacillus halodurans PO15: a mangrove bacterium from Poovar mangroves, India

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          Abstract

          Mangrove ecosystems are one of the most versatile habitats for microorganisms with a high potential for producing a variety of extracellular hydrolytic enzymes. In this study, bacteria with urease activity, enzymes that catalyze the hydrolysis of urea into carbon dioxide and ammonia, were isolated from mangrove sediments of Poovar (Trivandrum, India). Bacillus halodurans, strain PO15, isolated in this study with high urease (UA) activity (28 U/ml) was subjected to optimization using a Box-Behnken experimental design. Incubation variables included incubation period, pH, inoculation percentage and temperature. Significant factors identified based on the model were incubation period, pH, incubation temperature, and inoculum percentage; variations in these produced a tenfold increase in UA activity (295.80 U/ml). The specific activity of the purified UA enzyme was 62.34 U/mg and was found to be thermostable (active up to 60 °C). UA of B. halodurans PO15 has potential for microbial-induced biomineralization with a reduction of free Ca 2+ to about 82.8% ± 0.17%. The microbialinduced calcium precipitation (MICP) using the UA enzyme will potentially be beneficial in the process of biomineralization as well as for a variety of industrial applications.

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          Author and article information

          Journal
          MLST
          Marine Life Science & Technology
          Springer (China )
          2096-6490
          2662-1746
          01 May 2020
          15 April 2020
          : 2
          : 2
          : 194-202
          Affiliations
          1National Institute of Oceanography CSIR CSIR-NIO, Regional Centre, Kochi, Kerala 682018, India
          2School of Chemical and Biotechnology, SASTRA University, Thirumalasamudram, Thanjavur 613401, India
          Author notes
          *Corresponding author: Ammini Parvathi, E-mail: parvathi@ 123456nio.org
          Article
          s42995-020-00031-5
          10.1007/s42995-020-00031-5
          © 2020 Ocean University of China
          Product
          Self URI (journal-page): https://www.springer.com/journal/42995
          Categories
          Research Paper

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