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      Supplementing rooster sperm with Cholesterol-Loaded-Cyclodextrin improves fertility after cryopreservation.

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          Abstract

          Little is known about the effects of Cholesterol-Loaded Cyclodextrin (CLC) on post-thaw semen quality in chicken. The aim of the present study is to investigate the efficacy of CLC levels (0, 1, 2 and 3 mg/mL Schramm diluent) on post-thawed semen quality and fertility in two breeds of chicken Pradu Hang Dum (native chicken) and Rhode Island Red. Semen samples of each breed were pooled, divided into 4 aliquots and diluted with Schramm diluents, cooled to 5 °C when DMF was added (6% of final volume). Semen straws were subjected to cryopreservation using the liquid nitrogen vapor method. Post-thawed sperm motility, viability, acrosome integrity, mitochondrial function, and the Malondialdehyde (MDA) level were determined. The fertility of frozen semen was tested by inseminating laying hens. Post-thaw motility between Pradu Hang Dum and Rhode Island Red was no different; but Rhode Island Red had a higher semen viability and live cell intact acrosomes than Pradu Hang Dum (P < 0.05). The percentage of high functioning mitochondria in the Pradu Hang Dum was higher than the Rhode Island Red. CLC at 2 and 3 mg/mL supplementation was associated with improved viability of frozen semen; that is, acrosome integrity and mitochondrial function (P < 0.01), albeit having no effect on MDA levels. The sperm with 1 mg/mL CLC yielded a significantly better fertility (P < 0.01). CLC (1 mg/mL) improved the quality of frozen rooster semen. There was no interaction among breeds and CLC on post-thaw semen quality and fertility.

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          Author and article information

          Journal
          Cryobiology
          Cryobiology
          Elsevier BV
          1090-2392
          0011-2240
          February 2017
          : 74
          Affiliations
          [1 ] Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
          [2 ] Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand; Research and Development Network Center for Animal Breeding (Native Chicken), Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand. Electronic address: vthevi@kku.ac.th.
          Article
          S0011-2240(16)30481-3
          10.1016/j.cryobiol.2016.12.012
          28048968
          79da0b24-a13b-405a-bc8f-3044895b4c83
          History

          Fertility,Frozen semen,Rooster,Cholesterol Load Cyclodextrin (CLC),Breeds

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