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      Role of collapsin response mediator protein-2 in neurite outgrowth of PC12 cells.

      Neuroreport
      Animals, Blotting, Western, Cell Count, Cell Enlargement, Cell Line, Tumor, Cell Proliferation, Coculture Techniques, Humans, Intercellular Signaling Peptides and Proteins, deficiency, genetics, metabolism, Mice, Nerve Growth Factor, Nerve Tissue Proteins, Neural Cell Adhesion Molecules, Neurites, physiology, PC12 Cells, Rats, Transfection

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          Abstract

          Collapsin response mediator proteins (CRMPs) are involved in cell differentiation and axonal growth. In this study, we investigated neurite outgrowth and the expression of CRMP-2 in PC12 cells. Nondifferentiated PC12 cells hardly express CRMP-2, but the expression of CRMP-2 increases with neurite outgrowth. We established a stable CRMP-2-overexpressing PC12 cell line and found that PC12 cells, which constitutively overexpress CRMP-2, were unable to extend neurites after stimulation with the nerve growth factor or the neural cell adhesion molecule, a procedure that promotes neurite outgrowth in untransfected cells. In contrast, a PC12 cell line deficient in CRMP-2 extends neurites after the stimulation of nerve growth factor or neural cell adhesion molecule.

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