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      Piezoelectric immunochip coated with thin films of bacterial cellulose nanocrystals for dengue detection.

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          Abstract

          Low-cost piezoelectric devices, such as simple frequency monitoring quartz crystal microbalance (QCM) devices, have good clinical utility as fast diagnostic tools for the detection of several diseases. However, unspecific antigen recognition, poor molecular probe adsorption and the need for sample dilution are still common drawbacks that hinder their use in routine diagnosis. In this work, piezoelectric sensors were previously coated with thin films of bacterial cellulose nanocrystals (CN) to provide a more sensitive and adapted interface for the attachment of monoclonal immunoglobulin G (IgGNS1) and to favor specific detection of non-structural protein 1 (NS1) of dengue fever. The assembly of the immunochip surface was analyzed by atomic force microscopy (AFM) and the NS1 detection was followed by quartz crystal microbalance with (QCM-D) and without energy dissipation monitoring (QCM). The CN surface was able to immobilize 2.30±0.5mgm(-2) of IgGNS1, as confirmed by AFM topography and phase images along with QCM-D. The system was able to detect the NS1 protein in serum with only 10-fold dilution in the range of 0.01-10µgmL(-1) by both QCM and QCM-D. The limits of detection of the two devices were 0.1μgmL(-1) for QCM-D and 0.32μgmL(-1) for QCM. As a result, QCM-D and QCM apparatuses can be used to follow NS1 recognition and have good potential for more sensitive, fast and/or less expensive diagnostic assays for dengue.

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          Author and article information

          Journal
          Biosens Bioelectron
          Biosensors & bioelectronics
          Elsevier BV
          1873-4235
          0956-5663
          Jun 15 2017
          : 92
          Affiliations
          [1 ] Biochemistry and Molecular Biology Department, Federal University of Paraná, Centro Politécnico, CEP 81531-980 Curitiba, PR, Brazil; BioPol, Chemistry Department, Federal University of Paraná, P.B 19032, Centro Politécnico, CEP 81531-980 Curitiba, PR, Brazil.
          [2 ] BioPol, Chemistry Department, Federal University of Paraná, P.B 19032, Centro Politécnico, CEP 81531-980 Curitiba, PR, Brazil.
          [3 ] Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo (USP), CP 26077, 05513-970 São Paulo-SP, Brazil.
          [4 ] Biochemistry and Molecular Biology Department, Federal University of Paraná, Centro Politécnico, CEP 81531-980 Curitiba, PR, Brazil; BioPol, Chemistry Department, Federal University of Paraná, P.B 19032, Centro Politécnico, CEP 81531-980 Curitiba, PR, Brazil. Electronic address: mariarita.sierakowski@gmail.com.
          Article
          S0956-5663(17)30068-4
          10.1016/j.bios.2017.01.068
          28187298

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