For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
100
views
45
references
 Top references
cited by
171
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      51
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: not found

      Versatile P(acman) BAC Libraries for Transgenesis Studies in Drosophila melanogaster

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          We constructed Drosophila melanogaster BAC libraries with 21-kb and 83-kb inserts in the P(acman) system. Clones representing 12-fold coverage and encompassing more than 95% of annotated genes were mapped onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using ΦC31 integrase to rescue mutations. They can be modified through recombineering, for example to incorporate protein tags and assess expression patterns.

          Related collections

          Most cited references45

          • Record: found
          • Abstract: found
          • Article: not found

          An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases.

          Johannes Bischof, Robert Maeda, Monika Hediger … (2007)
          Germ-line transformation via transposable elements is a powerful tool to study gene function in Drosophila melanogaster. However, some inherent characteristics of transposon-mediated transgenesis limit its use for transgene analysis. Here, we circumvent these limitations by optimizing a phiC31-based integration system. We generated a collection of lines with precisely mapped attP sites that allow the insertion of transgenes into many different predetermined intergenic locations throughout the fly genome. By using regulatory elements of the nanos and vasa genes, we established endogenous sources of the phiC31 integrase, eliminating the difficulties of coinjecting integrase mRNA and raising the transformation efficiency. Moreover, to discriminate between specific and rare nonspecific integration events, a white gene-based reconstitution system was generated that enables visual selection for precise attP targeting. Finally, we demonstrate that our chromosomal attP sites can be modified in situ, extending their scope while retaining their properties as landing sites. The efficiency, ease-of-use, and versatility obtained here with the phiC31-based integration system represents an important advance in transgenesis and opens up the possibility of systematic, high-throughput screening of large cDNA sets and regulatory elements.
          Article 0 comments Cited 808 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            A gene expression atlas of the central nervous system based on bacterial artificial chromosomes.

            Shiaoching Gong, Chen Zheng, Martin Doughty … (2003)
            The mammalian central nervous system (CNS) contains a remarkable array of neural cells, each with a complex pattern of connections that together generate perceptions and higher brain functions. Here we describe a large-scale screen to create an atlas of CNS gene expression at the cellular level, and to provide a library of verified bacterial artificial chromosome (BAC) vectors and transgenic mouse lines that offer experimental access to CNS regions, cell classes and pathways. We illustrate the use of this atlas to derive novel insights into gene function in neural cells, and into principal steps of CNS development. The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously available to the neuroscience community.
            Article 0 comments Cited 603 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              The generic genome browser: a building block for a model organism system database.

              Lincoln D. Stein, Christopher John Mungall, ShengQiang Shu … (2002)
              The Generic Model Organism System Database Project (GMOD) seeks to develop reusable software components for model organism system databases. In this paper we describe the Generic Genome Browser (GBrowse), a Web-based application for displaying genomic annotations and other features. For the end user, features of the browser include the ability to scroll and zoom through arbitrary regions of a genome, to enter a region of the genome by searching for a landmark or performing a full text search of all features, and the ability to enable and disable tracks and change their relative order and appearance. The user can upload private annotations to view them in the context of the public ones, and publish those annotations to the community. For the data provider, features of the browser software include reliance on readily available open source components, simple installation, flexible configuration, and easy integration with other components of a model organism system Web site. GBrowse is freely available under an open source license. The software, its documentation, and support are available at http://www.gmod.org.
              Article 0 comments Cited 533 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-journal-id): 101215604
                Journal ID (pubmed-jr-id): 32338
                Journal ID (nlm-ta): Nat Methods
                Title: Nature methods
                ISSN (Print): 1548-7091
                ISSN (Electronic): 1548-7105
                Publication date Nihms-submitted: 4 May 2009
                Publication date (Print): June 2009
                Publication date PMC-release: 1 December 2009
                Volume: 6
                Issue: 6
                Pages: 431-434
                Affiliations
                [1 ]Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
                [2 ]Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 97420, USA
                [3 ]Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
                [4 ]Institute for Genomics and Systems Biology and Department of Human Genetics, University of Chicago, Chicago, IL 60637, USA
                [5 ]Children’s Hospital Oakland Research Institute, Oakland, CA 94609, USA
                [6 ]Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA
                [7 ]Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
                Author notes
                [* ]Correspondence (H.J.B.): E-mail, hbellen@ 123456bcm.tmc.edu ; Tel, 713-798-5272; Fax, 713-798-3694
                [8]

                These authors contributed equally to this work.

                Article
                Manuscript ID: nihpa112300
                DOI: 10.1038/nmeth.1331
                PMC ID: 2784134
                PubMed ID: 19465919
                SO-VID: 7b63660c-ec95-428f-9f5b-601f8f43ea37
                History
                Funding
                Funded by: National Human Genome Research Institute : NHGRI
                Funded by: National Institute of General Medical Sciences : NIGMS
                Funded by: Howard Hughes Medical Institute
                Award ID: U01 HG004264-01 ||HG
                Funded by: National Human Genome Research Institute : NHGRI
                Funded by: National Institute of General Medical Sciences : NIGMS
                Funded by: Howard Hughes Medical Institute
                Award ID: R01 GM080415-02 ||GM
                Funded by: National Human Genome Research Institute : NHGRI
                Funded by: National Institute of General Medical Sciences : NIGMS
                Funded by: Howard Hughes Medical Institute
                Award ID: ||HHMI_
                Categories
                Subject: Article

                ScienceOpen disciplines: Life sciences
                Data availability:
                ScienceOpen disciplines: Life sciences

                Comments

                Comment on this article

                scite_

                Similar content51

                See all similar

                Cited by161

                See all cited by