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      Effect of UVA and UVB Irradiation on the Metabolic Profile of Rabbit Cornea and Lens Analysed by HR-MAS 1H NMR Spectroscopy

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          Abstract

          Purpose:The aim of the study was to investigate the metabolic profiles of intact rabbit corneas and lenses exposed to UVA and UVB radiation by using high-resolution (HR) magic angle spinning (MAS) <sup>1</sup>H nuclear magnetic resonance (NMR) spectroscopy and pattern recognition methods. Methods:Adult albino rabbits were exposed to UVA (366 nm, 0.589 J/cm<sup>2</sup>) or UVB (312 nm, 1.667 J/cm<sup>2</sup>) radiation for 8 min, once a day for 5 days. Three days after the last irradiation day, samples of corneas and lenses were dissected. HR-MAS <sup>1</sup>H NMR spectroscopy combined with pattern recognition methods (principal component analysis and soft independent modelling of class analogy) and one-way ANOVA were applied to obtain metabolic information from intact corneal and lens tissue. Results: UVB irradiation caused statistically significant metabolic changes in the rabbit corneas. A decrease in metabolites as ascorbate (84%), myo-inositol (59%), hypotaurine (91%) and choline (76%) was observed. Exposure to UVA radiation caused no significant metabolic alteration in this tissue. The metabolic profile of the rabbit lenses showed no detectable changes after UVA or UVB exposure. Conclusions:The combination of HR-MAS <sup>1</sup>H NMR spectroscopy and multivariate methods proved effective to analyse intact corneal and lens tissue after exposure to UV radiation of different wavelengths. By avoiding extraction methods and obtaining complete metabolic profiles from one sample, HR-MAS <sup>1</sup>H NMR spectroscopy provided important information about metabolic alteration occurring in rabbit corneal and lens tissue after UV exposure.

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          Most cited references 27

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          Modified Spin-Echo Method for Measuring Nuclear Relaxation Times

           S. Meiboom,  D. Gill (1958)
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            NMR Spectroscopy of Biofluids

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              Corneal epithelium and UV-protection of the eye.

               A Ringvold (1998)
              To study UV-absorption and UV-induced fluorescence in the bovine corneal epithelium. Spectrophotometry and spectrofluorimetry. The corneal epithelium absorbs UV-B radiation mainly owing to its content of protein, RNA, and ascorbate. Some of the absorbed energy is transformed to the less biotoxic UV-A radiation by fluorescence. RNA and ascorbate reduce tissue fluorescence. The corneal epithelium acts as a UV-filter, protecting internal eye structures through three different mechanisms: (1) Absorption of UV-B roughly below 310 nm wavelength. (2) Fluorescence-mediated ray transformation to longer wavelengths. (3) Fluorescence reduction. The extremely high ascorbate concentration in the corneal epithelium has a key role in two of these processes.
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                Author and article information

                Journal
                ORE
                Ophthalmic Res
                10.1159/issn.0030-3747
                Ophthalmic Research
                S. Karger AG
                0030-3747
                1423-0259
                2006
                February 2006
                01 March 2006
                : 38
                : 2
                : 105-114
                Affiliations
                Departments of aNeuroscience, Faculty of Medicine, and bCirculation and Medical Imaging, Norwegian University of Science and Technology (NTNU), Trondheim, Norway; cInstitute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
                Article
                90511 Ophthalmic Res 2006;38:105–114
                10.1159/000090511
                16374053
                © 2006 S. Karger AG, Basel

                Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

                Page count
                Figures: 5, References: 41, Pages: 10
                Categories
                Original Paper

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