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      Effect of UVA and UVB Irradiation on the Metabolic Profile of Rabbit Cornea and Lens Analysed by HR-MAS 1H NMR Spectroscopy

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          Purpose:The aim of the study was to investigate the metabolic profiles of intact rabbit corneas and lenses exposed to UVA and UVB radiation by using high-resolution (HR) magic angle spinning (MAS) <sup>1</sup>H nuclear magnetic resonance (NMR) spectroscopy and pattern recognition methods. Methods:Adult albino rabbits were exposed to UVA (366 nm, 0.589 J/cm<sup>2</sup>) or UVB (312 nm, 1.667 J/cm<sup>2</sup>) radiation for 8 min, once a day for 5 days. Three days after the last irradiation day, samples of corneas and lenses were dissected. HR-MAS <sup>1</sup>H NMR spectroscopy combined with pattern recognition methods (principal component analysis and soft independent modelling of class analogy) and one-way ANOVA were applied to obtain metabolic information from intact corneal and lens tissue. Results: UVB irradiation caused statistically significant metabolic changes in the rabbit corneas. A decrease in metabolites as ascorbate (84%), myo-inositol (59%), hypotaurine (91%) and choline (76%) was observed. Exposure to UVA radiation caused no significant metabolic alteration in this tissue. The metabolic profile of the rabbit lenses showed no detectable changes after UVA or UVB exposure. Conclusions:The combination of HR-MAS <sup>1</sup>H NMR spectroscopy and multivariate methods proved effective to analyse intact corneal and lens tissue after exposure to UV radiation of different wavelengths. By avoiding extraction methods and obtaining complete metabolic profiles from one sample, HR-MAS <sup>1</sup>H NMR spectroscopy provided important information about metabolic alteration occurring in rabbit corneal and lens tissue after UV exposure.

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          Most cited references 27

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          Modified Spin-Echo Method for Measuring Nuclear Relaxation Times

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              Corneal epithelium and UV-protection of the eye.

               A Ringvold (1998)
              To study UV-absorption and UV-induced fluorescence in the bovine corneal epithelium. Spectrophotometry and spectrofluorimetry. The corneal epithelium absorbs UV-B radiation mainly owing to its content of protein, RNA, and ascorbate. Some of the absorbed energy is transformed to the less biotoxic UV-A radiation by fluorescence. RNA and ascorbate reduce tissue fluorescence. The corneal epithelium acts as a UV-filter, protecting internal eye structures through three different mechanisms: (1) Absorption of UV-B roughly below 310 nm wavelength. (2) Fluorescence-mediated ray transformation to longer wavelengths. (3) Fluorescence reduction. The extremely high ascorbate concentration in the corneal epithelium has a key role in two of these processes.

                Author and article information

                Ophthalmic Res
                Ophthalmic Research
                S. Karger AG
                February 2006
                01 March 2006
                : 38
                : 2
                : 105-114
                Departments of aNeuroscience, Faculty of Medicine, and bCirculation and Medical Imaging, Norwegian University of Science and Technology (NTNU), Trondheim, Norway; cInstitute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
                90511 Ophthalmic Res 2006;38:105–114
                © 2006 S. Karger AG, Basel

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                Page count
                Figures: 5, References: 41, Pages: 10
                Original Paper


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