Computerized image analysis of seedlings to evaluate broccoli seed vigor

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Abstract

Abstract: The computerized image analysis of seedlings constitutes an efficient technique to evaluate the quality of seed lots, since it is quick in responding, has a simple execution and reproduction. The goal of this research was to verify the efficiency of the computer image analysis of seedlings to detect vigor differences among broccoli seed lots and compare them with other traditionally used vigor tests. Computerized image analysis were performed by the Seed Vigor Imaging System (SVIS®), using 3-day-old seedlings and the results were compared to germination at first count, seedling emergence, accelerated aging and electrical conductivity tests. Evaluations were performed in three experimental periods (before storage, 6 and 12 months of storage at 20 °C and 45-50% RH). A completely randomized design with eight replications of 25 seeds was used. Data were submitted to analysis of variance and means were compared by Scott-Knott test at 5% error probability. It is concluded that it is possible to detect vigor differences among broccoli seed lots by the computerized image analysis of seedlings with SVIS®, similarly to traditional vigor tests.

Translated abstract

Resumo: A análise computadorizada de imagens de plântulas se apresenta como técnica eficiente na avaliação da qualidade de lotes de sementes de várias espécies, sendo um método de rápida resposta, com metodologia de simples execução e reprodução. Objetivou-se com esta pesquisa verificar a possibilidade de utilização da análise computadorizada de imagens de plântulas na detecção de possíveis diferenças de vigor entre lotes de sementes de brócolis e comparar com os testes de vigor tradicionalmente utilizados. Foram utilizadas sementes de dois cultivares, Piracicaba Precoce e Ramoso Santana, representados por dez lotes de cada. A pesquisa consistiu da utilização do sistema computadorizado de imagens de plântulas (Seed Vigor Imaging System - SVIS®) para detectar possíveis diferenças de vigor de sementes de brócolis durante o armazenamento, por doze meses, com três avaliações (antes do armazenamento, 6 e 12 meses de armazenamento a 20 ºC e 45-50% UR). O delineamento experimental utilizado foi o inteiramente casualizado, com oito repetições de 25 sementes. Os dados referentes às avaliações foram submetidos à análise de variância e as comparações de médias realizadas pelo teste de Scott-Knott ao nível de 5% de probabilidade de erro. É possível detectar diferenças de vigor entre lotes de sementes de brócolis pela análise computadorizada de imagens de plântulas, com o SVIS®, de forma semelhante aos testes tradicionais de vigor.

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Seed vigor testing: an overview of the past, present and future perspective

Julio Marcos-Filho (2015)

The assessment of seed vigor has many important implications to the seed industry as a basic monitoring of seed physiological potential during different phases of seed production and a support for strategic decisions regarding the selection of high quality seedlots to meet the consumer demand. The potential attributes of seed vigor as a fundamental physiological seed characteristic and its association with field stand establishment and crop productivity has been worldwide recognized from the 1960s onward. This led to the diversification of research approaches involving the synchronization of different physiological characteristics and events that determine the potential for high performance during seed storage and after sowing. The basic objective of vigor testing is to provide a consistent identification of differences in physiological potential among seedlots of commercial value and this represents a more sensitive parameter than the germination test. There are various procedures to assess seed vigor including those that directly or indirectly evaluate seed metabolic state or identify seed tolerance to specific stress(es). At the same time, in more recent years, new knowledge has come from molecular biology, biotechnology, biophysics and seed and seedling imaging analyses; these approaches has been important complements to traditional seed research.. This text has the purpose to emphasize the role of Brazilian research in seed vigor, whose competence is internationally recognized, and to provide an overview of the evolution of knowledge also attempting to highlight events that contributed to the advancement of research on the subject.