Pulsed-wave Doppler analysis of tricuspid inflow and hepatic venous flow allows the determination of multiple parameters of right-ventricular (RV) diastolic function. This study sought to evaluate the influence of heart rate, age and tricuspid regurgitation on Doppler parameters of RV diastolic performance. We studied 80 subjects (20 normal and 60 with RV pathology) with Doppler recordings of the tricuspid inflow and hepatic vein. All tricuspid Doppler parameters were dependent on heart rate, age or regurgitation severity as were the hepatic vein S wave, S/D ratio and systolic filling fraction. The atrial reversal velocity in the hepatic vein Doppler tracing was not affected by age, heart rate or regurgitation. In addition, the inspiratory increase in this parameter was significantly elevated in RV pathologic states. This study demonstrates that most Doppler echocardiographic parameters of RV diastolic performance are dependent on age, tricuspid regurgitation or heart rate.