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A palmtop-sized microfluidic cell culture system driven by a miniaturized infusion pump.

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      Abstract

      A palmtop-sized microfluidic cell culture system is presented. The system consists of a microfluidic device and a miniaturized infusion pump that possesses a reservoir of culture medium, an electrical control circuit, and an internal battery. The footprint of the system was downsized to 87 × 57 mm, which is, to the best of our knowledge, the smallest integrated cell culture system. Immortalized human microvascular endothelial cells (HMEC-1) and human umbilical vein endothelial cells (HUVEC) were cultured in the system. HMEC-1 in the system proliferated at the same speed as cells in a microchannel perfused by a syringe pump and cells in a culture flask. HUVEC in the system oriented along the direction of the fluid flow. Claudin-5, a tight junction protein, was localized along the peripheries of the HUVEC. We expect that the present system is applicable to various cell types as a stand-alone and easy-to-use system for microfluidic bioanalysis.

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      Affiliations
      [1 ] Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Mejirodai, Bunkyo-ku, Tokyo, Japan.
      Journal
      Electrophoresis
      Electrophoresis
      1522-2683
      0173-0835
      Jul 2012
      : 33
      : 12
      22740461
      10.1002/elps.201100691
      © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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