Characterization of sensory neuron membrane proteins (SNMPs) in cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae).

Abstract

Sensory neuron membrane proteins (SNMPs) play a critical role in insect chemosensory system. Previously, three SNMPs were identified, characterized and functionally investigated in a lepidopteran model insect, Bombyx mori. However, whether these results are consistent across other lepidopteran species are unknown. Here genome and transcriptome data analysis, expression profiling, quantitative real-time PCR (qRT-PCR) and the yeast hybridization system were utilized to examine snmp genes of Helicoverpa armigera, one of the most destructive lepidopteran pests in cropping areas. In silico expression and qRT-PCR analyses showed that, just as the B. mori snmp genes, H. armigera snmp1 (Harmsnmp1) is specifically expressed in adult antennae. Harmsnmp2 is broadly expressed in multiple tissues including adult antennae, tarsi, larval antennae and mouthparts. Harmsnmp3 is specifically expressed in larval midguts. Further RNAseq analysis suggested that the expression levels of Harmsnmp2 and Harmsnmp3 differed significantly depending on the plant species on which the larvae fed, indicating they may be involved in plant-feeding behaviours. Yeast hybridization results revealed a protein-protein interaction between HarmSNMP1 and the sex pheromone receptor, HarmOR13. This study demonstrated that SNMPs may share same functions and mechanisms in different lepidopteran species, which improved our understanding of insect snmp genes and their functions in lepidopterans.