Sir,
Gonorrhoea is one of the most common sexually transmitted infections (STIs) in developing
countries and is a global public health problem
1
2.
Gonorrhoea is an easily curable STI, but if remained undetected, untreated infections
and co-infections can lead to complications like pelvic inflammatory disease, ectopic
pregnancy, tubal factor infertility, adverse pregnancy outcomes in females, and testicular
and prostate infections and infertility in males. Also, asymptomatic patients, unaware
of their infection, may serve as a reservoir of infection to their partners. Moreover,
STIs including non-ulcerative STIs like gonorrhoea potentially increase the risk of
both transmission and acquisition of human immunodeficiency virus (HIV)
3.
Socio-economic factors like women’s emancipation, permissiveness, homosexuality, population
migration and increased availability of diagnostic facilities have resulted in increasing
these STI rates. However, rate of gonorrhoea and other non-ulcerative STIs are difficult
to determine because clinical presentation is not specific enough and facilities,
materials, or personnel for laboratory based diagnosis are inadequate. Moreover, there
is lack of reporting mechanism and reluctance to report STIs to public health authorities.
There are many reports on co-infection between gonorrhoea and chlamydia infection.
Little information is available on co-infection of gonorrhoea with other STIs. Keeping
this aspect in mind we carried out retrospective analysis of six year data to evaluate
the gonococcal infection rate in patients attending male and female STD clinic of
the Regional STD Teaching, Training & Research Centre, Safdarjang Hospital, New Delhi,
and analysed its association with other ulcerative (syphilis, chancroid, herpes, donovanosis),
non-ulcerative STIs [chlamydiasis, trichomoniasis, bacterial vaginosis (BV), candidiasis]
and HIV infection. This Regional STD Centre has been monitoring the trends of antimicrobial
resistance in Neisseria gonorrhoeae and prevalence of STIs4–7.
A total of 5871 records from male and female patients who visited the Centre between
January 2003 to December 2008 were analyzed. Urethritis related symptoms and genital
ulcer, and cervical/vaginal discharge and genital ulcer were the main eligibility
criteria for males and females, respectively for inclusion in the study. Demographic
information, including age, race, gender, and symptoms was also collected.
Standard laboratory procedures were used for the diagnosis of gonorrhoea and other
STIs8
9. Direct urethral/cervical smear and culture on chocolate agar and saponin-lysed
blood agar with vancomycin, colistin, nystatin, trimethoprim (VCNT) supplement were
carried out to diagnose N. gonorrhoeae and the isolates were confirmed by standard
methods. For syphilis, dark field examination, VDRL (Venereal Disease Research Laboratory)
test (antigen from Serologist to Govt. of India, Kolkata), Treponema pallidum haemagglutination
assay (Plasmatec TPHA test kit, Hansard Diagnostics, United Kingdom) in VDRL reactive
cases and fluorescent treponemal antibody absorption (FTA-ABS) test using FTA-Abs
IgG and IgM IFA (Viro-Immun Labor-Diagnostika Gmbh, Oberursel & Virgo, Calbiotec,
USA) in sera giving discrepant results in the above two tests were carried out. For
herpes progenitalis ulcer smear and IgM HSV-2 ELISA; for donovanosis tissue smears;
and for chancroid smear and culture using two medium i.e., GC agar base with iso-vitalex,
vancomycin and fetal calf serum and Mueller Hinton agar with iso-vitalex, vancomycin
and fetal calf serum from ulcer base were performed. Tests for chlamydial infections
included antigen detection by ELISA (Bio-Rad Laboratories, USA) and direct fluorescent
antibody (DFA) test (Immuno FA, Orgenics, Israel) and Gram-stained urethral/cervical
smear showing four or more polymorphonuclear cells on high-power examination. For
diagnosis of trichomoniasis, a direct wet mount examination and culturing on Whittington
media; for candidiasis, direct Gram stained smear examination and culture on Saboraud’s
dextrose agar, followed by culture confirmation by germ tube test; and for BV, physiological
tests (pH test >4 and amine tests) and vaginal Gram stained smear (interpretation
following Nugent’s criteria), were performed. Besides these tests, presence of HIV
1 and 2 antibodies were determined in the patients by ELISA/Rapid tests, using NACO
approved kits, following NACO guidelines
10 after pretest counselling, and written informed consent, followed by post-test
counselling.
The differences in percentages were statistically compared by determining standard
error of proportions, tested for significance by using Z test. The presence of co-infections
was compared by chi-square test.
During the study period, a total of 353 gonorrhoea cases were detected and throughout
the study period, gonorrhoea rate was more in males than females. Of the total 353
cases based on culture positivity, 315 males were harbouring gonorrhoea infections,
whereas infected females were only 38. The presence of gonorrhoea in males was observed
to be higher in 2003-2006 (varying from 9.3 to 12.1%), dropping in 2007 (6.4%). This
decrease from 9.3 per cent in 2003 to 6.4 per cent in 2007 was found to be statistically
significant (P<0.001). However, in 2008 there was a significant substantial rise to
12.4 per cent (P<0.001). The occurrence of gonorrhoea was lower in females varying
from 0.4 to 3.8 per cent. Patients in 21-30 yr age group accounted for majority of
cases of gonorrhoea (56.4%) and 20.7 per cent belonged to 31-40 yr. This was followed
by 15-20 yr age group (13.1%) and 41-50 yr (7.4%). Eight cases (2.2%) belonged to
51-70 yr age group.
Among ulcerative STIs, presence of syphilis was highest (36.9%), followed by herpes
progenitalis (15.4%). A few cases were reported to have chancroid (0.9%) and donovanosis
(0.1%). Of the non-ulcerative STIs, infection by Candida albicans was most frequently
observed (23.3%) while BV, C. trachomatis, Trichomonas vaginalis accounted for 5.3,
4.2 and 1.8 per cent, respectively. All the ulcerative STIs and genital warts were
more common in males. Rate of genital warts and HIV infection was found to be 11.0
and 9.7 per cent.
The Table shows the distribution of various other STIs by aetiological diagnosis among
N. gonorrhoeae culture positive cases. Overall, 14.4 per cent (51/353) gonorrhoea
patients had co-infection with another STIs. Among the male gonorrhoea cases, the
most common co-infection was syphilis i.e., 7.0 per cent followed by HIV 2.2 per cent
(P<0.01). Other minor co-infections were with C. albicans 0.9 per cent, C. trachomatis
0.9 per cent, chancroid 0.3 per cent and herpes 0.3 per cent (P<0.01).
Table
Co-infection of gonorrhoea with other sexually transmitted infections (STIs)
STI
Male No.(%)
Female No.(%)
Total No.(%)
Total N. gonorrhoeae culture positive cases
315
38
353
Syphilis
22 (7.0)
0
22 (6.3)
Chancroid
1 (0.3)
0
1 (0.3)
Ulcerative STIs
Herpes
1 (0.3)
0
1 (0.3)
Donovanosis
0
0
0
Trichomonas vaginalis
0
1 (2.6)
1 (0.3)
Candida albicans
3 (0.9)
6 (15.8)
9 (2.5)
Non-ulcerative STIs
Bacterial vaginosis
Not applicable
6 (15.8)
6 (1.7)
Chlamydia trachomatis
3 (0.9)
1 (2.6)
4 (1.1)
Genital warts
0
0
0
Other STIs
HIV
7 (2.2)
0
7 (2.0)
Total co-infections
37 (11.7)
14 (36.8)
51 (14.4)
Among the comparatively low prevalent gonorrhoea positive females, no co-infections
were found for ulcerative STIs. The highest association in females was with non-ulcerative
STIs such as bacterial vaginosis, and C. albicans i.e., 15.8 per cent each (P<0.05).
Other statistically insignificant co-infections included C. trachomatis and T. vaginalis
2.6 per cent each. Co-infection with more than two STIs was rare.
The occurrence of gonorrhoea in patients attending this centre has changed since 19906.
It remained constant around 13 per cent from 1990 to 1997 and thereafter, there was
a significant rise to 19.4 per cent in 2001 and again decreased to 15.4 per cent in
20026. Gonorrhoea rates in the present study dropped from 12.1 per cent in 2004 to
6.4 per cent in 2007. This drop may either be due to actual decreasing rate of gonorrhoea
over the years or due to the fact that gradually less number of patients were reporting
to the clinic because of easy availability of antimicrobials as a part of syndromic
management of STIs in peripheral and private health set ups.
Our study showed maximum cases in the 21-30 yr age group, and among males. The subjects
as found from patient’s history were mostly unmarried and hence more prone to visit
commercial sex workers. The data support earlier consensus that young adults and adolescents
should constitute priority target group in STD control programme.
Gonorrhoea rate was quite low in females in the present study. It may be because most
of the female patients were referred from Gynaecology clinic to STD clinic after they
did not respond to syndromic management of genital discharges. Besides, most of the
female patients were not having purulent/muco purulent cervical discharge and thus
were not cases of gonorrhoea. In contrast, in Mumbai study11, prevalence was 9.7 per
cent in women attending STD clinic. Gonococcal urethritis rate was observed to be
7 per cent in STD clinic in Assam Medical College Hospital, Dibrugarh12.
In the present study, in only 4 of 353 (1.1%) cases of gonorrhoea, co-infection with
C. trachomatis was observed. This finding is in contrast to high association between
N. gonorrhoeae and C. trachomatis (27.2%) observed in a study from Mumbai, India11
and as also (range, 9-67%) found in other studies from United States13. In a recent
study from Italy
14, co-infection with C. trachomatis was detected in 46 per cent patients. Otherwise
also, C. trachomatis infection prevalence was low (4.5%) in the present study in comparison
to above studies resulting in lesser rate of co-infection. This may have been due
to use of less sensitive enzyme immunoassay, and DFA test. The newer, more sensitive
nucleic acid amplification tests [such as polymerase chain reaction (PCR)] being more
expensive could not be used in this study.
Prevalence of BV among gonorrhoea patients was 15.8 per cent in contrast to 3.2 per
cent in Indonesia15 and 2.2 per cent in USA
16. In both above, population studied was pregenant women. In a community based study
from India17, association with BV was nil. Association between N. gonorrhoeae and
Trichomonas vaginalis has been observed to be 1, 10 and 11.7 per cent in Indiana,
United States and Mumbai respectively11
18
19. This is quite high in comparison to our study (0.3%). High sensitivity of PCR
used in these studies in comparison to culture could have attributed to this. This
was explained by Seña et al
19 that urine cultures detected T. vaginalis infection in 8 per cent, whereas urine
PCR demonstrated infection in 70 per cent.
Our study showed the highest rate of co-infection among gonorrhoea positive cases
to be with syphilis (6.3%). Similarly, Bozicevic et al
20 found it to be 9 per cent in symptomatic heterosexual men. Recently, high rate
of co-infection with HIV i.e., 17 per cent were detected in patients infected with
N. gonorrhoeae in Kenya21 in comparison to our study (2%). In Mumbai study
11, co-infection with HIV was observed to be very high (92.3%), while it was nil in
a STD centre in North Eastern State of India22.
In this study, only two patients were observed to have multiple co-infections probably
because in gonorrhoea the infection is not as prolonged and the symptomatic patients
tend to seek treatment at the early stage.
Differences in the method used for diagnosis of STIs and the size and type of population
studied may lead to variations in the co-infection rate. High co-infection rate (14.4%)
of gonorrhoea with other STIs from 2003-2008 highlight the considerable burden of
the disease indicating that appropriate screening measures for STIs must be widely
and consistently implemented so as to assure prompt and effective treatment for infected
persons and their sexual partners leading to reductions in disease burden.