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      About Notiophilus Duméril, 1806 (Coleoptera, Carabidae): Species delineation and phylogeny using DNA barcodes

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      Deutsche Entomologische Zeitschrift
      Pensoft Publishers

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          Abstract

          The genus Notiophilus Duméril, 1806 is a distinctive taxon of small, diurnal and morphologically similar beetles exhibiting large eyes and widened second elytral intervals. In this study we analysed the effectiveness of DNA barcodes to discriminate 67 specimens that represent 8 species of Notiophilus from Central Europe. Interspecific K2P distances below 2.2% were found for N. biguttatus (Fabricius, 1779) and N. quadripunctatus Dejean, 1826, whereas intraspecific distances with values > 2.2% were revealed for N. rufipes Curtis, 1829. An additional phylogenetic analysis of all available species revealed a close relationship of N. directus Casey, 1920, N. semistriatus Say, 1823, N. simulator Fall, 1906 and N. sylvaticus Dejean, 1831, possibly indicating a radiation of these species in North America. Low support values of most other nodes, however, do not allow additional phylogenetic conclusions.

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          Wolbachia: master manipulators of invertebrate biology.

          Wolbachia are common intracellular bacteria that are found in arthropods and nematodes. These alphaproteobacteria endosymbionts are transmitted vertically through host eggs and alter host biology in diverse ways, including the induction of reproductive manipulations, such as feminization, parthenogenesis, male killing and sperm-egg incompatibility. They can also move horizontally across species boundaries, resulting in a widespread and global distribution in diverse invertebrate hosts. Here, we review the basic biology of Wolbachia, with emphasis on recent advances in our understanding of these fascinating endosymbionts.
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            An inexpensive, automation-friendly protocol for recovering high-quality DNA

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              Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring

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                Author and article information

                Journal
                Deutsche Entomologische Zeitschrift
                DEZ
                Pensoft Publishers
                1860-1324
                1435-1951
                May 29 2019
                May 29 2019
                : 66
                : 1
                : 63-73
                Article
                10.3897/dez.66.34711
                7ce80093-23d6-4b83-b136-f00173a251c1
                © 2019

                http://creativecommons.org/licenses/by/4.0/

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